Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes

Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes

Abstract Background The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult. Results We introduce a working pipeline for expression, purification a...

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Bibliographic Details
Main Authors:	Jiang Xu, Xiao Lei, Ao Li, Jun Li, Shuxing Li, Lin Chen
Format:	Article
Language:	English
Published:	BMC 2024-02-01
Series:	Microbial Cell Factories
Subjects:	Three finger protein E. coli recombinant expression Inclusion body refolding Disulfide bond formation Oxidation refolding
Online Access:	https://doi.org/10.1186/s12934-024-02316-1

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