Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis
The process of papain assisted hot water (WP), cellulase assisted hot water (WC), papain mixed cellulase assisted hot water (WPC), ultrasonic assisted papain (PU), and ultrasonic assisted cellulase (CU) for Patriniae polysaccharides (HPs) extraction were optimized using the indicate of polysaccharid...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522300922X |
| _version_ | 1797390481342070784 |
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| author | Heping Hui Xuejun Wang Nianwu He Jianfei Liu Siyuan Tan Yiling Zhang Lin Chen Xiao-dong Li |
| author_facet | Heping Hui Xuejun Wang Nianwu He Jianfei Liu Siyuan Tan Yiling Zhang Lin Chen Xiao-dong Li |
| author_sort | Heping Hui |
| collection | DOAJ |
| description | The process of papain assisted hot water (WP), cellulase assisted hot water (WC), papain mixed cellulase assisted hot water (WPC), ultrasonic assisted papain (PU), and ultrasonic assisted cellulase (CU) for Patriniae polysaccharides (HPs) extraction were optimized using the indicate of polysaccharide yield, respectively. Then, the physicochemical characteristics, antioxidant and hypoglycemic activities of HPs were compared, with an additional correlation analysis between the characteristics and activities of HPs. The results showed that CU demonstrated the highest extraction rate at 30.50 % among all methods. Compared to previous hot water extraction (HW), the enzyme-assisted extraction increased molecular weight, reducing sugar, mannose, and glucose content of HPs, while ultrasound-assisted extraction lowered mannose and glucose content of HPs, and increased reducing sugar content of HPs. All HPs showed concentration-dependent hypoglycemic and antioxidant activities. Correlation analysis revealed that HPs with moderate galactose content, higher reducing sugar, protein, glucose, mannose, xylose, and molecular weight polysaccharide content would exhibit superior hypoglycemic and antioxidant activities, with CU-HPs expressing these excellent characteristics. Together with the exceptional polysaccharide yield, CU was a promising strategy to extract HPs, whose optimum process were: enzyme addition 2.5 %, solid–liquid ratio 1:25, ultrasonic power 150 W, ultrasonic time of 50 min. |
| first_indexed | 2024-03-08T23:12:30Z |
| format | Article |
| id | doaj.art-603ad2c997cc4268be7107af2a4516c1 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-08T23:12:30Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-603ad2c997cc4268be7107af2a4516c12023-12-15T07:23:36ZengElsevierArabian Journal of Chemistry1878-53522024-01-01171105460Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysisHeping Hui0Xuejun Wang1Nianwu He2Jianfei Liu3Siyuan Tan4Yiling Zhang5Lin Chen6Xiao-dong Li7College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, China; Corresponding authors.College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, China; Corresponding authors.College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, ChinaLanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, ChinaCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, ChinaCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, ChinaCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, ChinaInstitute of Chinese Materia Medica, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730010, ChinaThe process of papain assisted hot water (WP), cellulase assisted hot water (WC), papain mixed cellulase assisted hot water (WPC), ultrasonic assisted papain (PU), and ultrasonic assisted cellulase (CU) for Patriniae polysaccharides (HPs) extraction were optimized using the indicate of polysaccharide yield, respectively. Then, the physicochemical characteristics, antioxidant and hypoglycemic activities of HPs were compared, with an additional correlation analysis between the characteristics and activities of HPs. The results showed that CU demonstrated the highest extraction rate at 30.50 % among all methods. Compared to previous hot water extraction (HW), the enzyme-assisted extraction increased molecular weight, reducing sugar, mannose, and glucose content of HPs, while ultrasound-assisted extraction lowered mannose and glucose content of HPs, and increased reducing sugar content of HPs. All HPs showed concentration-dependent hypoglycemic and antioxidant activities. Correlation analysis revealed that HPs with moderate galactose content, higher reducing sugar, protein, glucose, mannose, xylose, and molecular weight polysaccharide content would exhibit superior hypoglycemic and antioxidant activities, with CU-HPs expressing these excellent characteristics. Together with the exceptional polysaccharide yield, CU was a promising strategy to extract HPs, whose optimum process were: enzyme addition 2.5 %, solid–liquid ratio 1:25, ultrasonic power 150 W, ultrasonic time of 50 min.http://www.sciencedirect.com/science/article/pii/S187853522300922XHerba Patriniae polysaccharideProcess optimizationPhysicochemical characteristicsAntioxidant and hypoglycemic activityCorrelation analysis |
| spellingShingle | Heping Hui Xuejun Wang Nianwu He Jianfei Liu Siyuan Tan Yiling Zhang Lin Chen Xiao-dong Li Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis Arabian Journal of Chemistry Herba Patriniae polysaccharide Process optimization Physicochemical characteristics Antioxidant and hypoglycemic activity Correlation analysis |
| title | Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis |
| title_full | Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis |
| title_fullStr | Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis |
| title_full_unstemmed | Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis |
| title_short | Process optimization and effect of different extraction methods on the characteristics and activities of Herba Patriniae polysaccharides: A correlation analysis |
| title_sort | process optimization and effect of different extraction methods on the characteristics and activities of herba patriniae polysaccharides a correlation analysis |
| topic | Herba Patriniae polysaccharide Process optimization Physicochemical characteristics Antioxidant and hypoglycemic activity Correlation analysis |
| url | http://www.sciencedirect.com/science/article/pii/S187853522300922X |
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