Measurement of Protein Mobility in Listeria monocytogenes Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion

Measurement of Protein Mobility in Listeria monocytogenes Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion

Protein mobility in the cytoplasm is essential for cellular functions, and slow diffusion may limit the rates of biochemical reactions in the living cell. Here, we determined the apparent lateral diffusion coefficient (DL) of GFP in Listeria monocytogenes as a function of osmotic stress, temperature...

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Bibliographic Details
Main Authors: Buu Minh Tran, Haritha Prabha, Aditya Iyer, Conor O’Byrne, Tjakko Abee, Bert Poolman
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Microbiology
Subjects:
protein mobility
lateral diffusion
Listeria monocytogenes
osmotic stress
fluorescence recovery after photobleaching
macromolecular crowding
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.640149/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.640149/full

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