Protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo non-canonical amino acid tagging

Protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo non-canonical amino acid tagging

Summary: The fluorescent non-canonical amino acid tagging (FUNCAT) technique has been used to visualize newly synthesized proteins in cell lines and tissues. Here, we present a protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo FUNCAT. We describe steps...

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Bibliographic Details
Main Authors: Mehdi Hooshmandi, Calvin Wong, Kevin C. Lister, Nicole Brown, Weihua Cai, David Ho-Tieng, Patricia Stecum, Thomas Backman, Elie Kostantin, Arkady Khoutorsky
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Microscopy
Model Organisms
Neuroscience
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007426

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http://www.sciencedirect.com/science/article/pii/S2666166723007426

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