Multifocal imaging for precise, label-free tracking of fast biological processes in 3D

Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.

Bibliographic Details
Main Authors: Jan N. Hansen, An Gong, Dagmar Wachten, René Pascal, Alex Turpin, Jan F. Jikeli, U. Benjamin Kaupp, Luis Alvarez
Format: Article
Language:English
Published: Nature Portfolio 2021-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-24768-4