Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-07-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-021-24768-4 |