Preparation, characterization, and insulin sensitivity of isolated swine adipocytes: comparison with adipose tissue slices.
The technique of Rodbell (J. Biol. Chem. 239: 375) was modified considerably in order to isolate swine adipocytes without rupturing large cells. Cell size and diameter distributions were the same for adipocytes fixed with OsO4 following isolation with collagenase and adipoctes liberated from OsO4-fi...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1981-09-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520406625 |