Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection
Abstract Background Understanding pathogenic mechanisms is imperative for developing novel treatment to the tuberculosis, an important public health burden worldwide. Recent studies demonstrated that host cholesterol levels have implications in the establishment of Mycobacterium tuberculosis (M. tub...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-05-01
|
| Series: | BMC Immunology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12865-020-00356-x |
| _version_ | 1818305623171792896 |
|---|---|
| author | Jinrui Xu Yanbing Zhou Yi Yang Cuiping Lv Xiaoming Liu Yujiong Wang |
| author_facet | Jinrui Xu Yanbing Zhou Yi Yang Cuiping Lv Xiaoming Liu Yujiong Wang |
| author_sort | Jinrui Xu |
| collection | DOAJ |
| description | Abstract Background Understanding pathogenic mechanisms is imperative for developing novel treatment to the tuberculosis, an important public health burden worldwide. Recent studies demonstrated that host cholesterol levels have implications in the establishment of Mycobacterium tuberculosis (M. tuberculosis, Mtb) infection in host cells, in which the intracellular cholesterol-mediated ATP-binding cassette transporters (ABC-transporters) and cholesterol acyltransferase1 (ACAT1) exhibited abilities to regulate macrophage autophagy induced by Mycobacterium bovis bacillus Calmette–Guérin (BCG). Results The results showed that a down-regulated expression of the ABC-transporters and ACAT1 in primary bovine alveolar macrophages (AMs) and murine RAW264.7 cells in response to a BCG infection. The inhibited expression of ABC-transporters and ACAT1 was associated with the reduction of intracellular free cholesterol, which in turn induced autophagy in macrophages upon to the Mycobacterial infection. These results strongly suggest an involvement of ABC-transporters and ACAT1 in intracellular cholesterol-mediated autophagy in AMs in response to BCG infection. Conclusion This study thus provides an insight into into a mechanism by which the cholesterol metabolism regulated the autophagy in macrophages in response to mycobacterial infections. |
| first_indexed | 2024-12-13T06:29:32Z |
| format | Article |
| id | doaj.art-609e5eb0b4aa4d898ca5e9554d9b4bb5 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-13T06:29:32Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-609e5eb0b4aa4d898ca5e9554d9b4bb52022-12-21T23:56:39ZengBMCBMC Immunology1471-21722020-05-0121111110.1186/s12865-020-00356-xInvolvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infectionJinrui Xu0Yanbing Zhou1Yi Yang2Cuiping Lv3Xiaoming Liu4Yujiong Wang5Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternKey Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternKey Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternKey Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternKey Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternKey Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the WesternAbstract Background Understanding pathogenic mechanisms is imperative for developing novel treatment to the tuberculosis, an important public health burden worldwide. Recent studies demonstrated that host cholesterol levels have implications in the establishment of Mycobacterium tuberculosis (M. tuberculosis, Mtb) infection in host cells, in which the intracellular cholesterol-mediated ATP-binding cassette transporters (ABC-transporters) and cholesterol acyltransferase1 (ACAT1) exhibited abilities to regulate macrophage autophagy induced by Mycobacterium bovis bacillus Calmette–Guérin (BCG). Results The results showed that a down-regulated expression of the ABC-transporters and ACAT1 in primary bovine alveolar macrophages (AMs) and murine RAW264.7 cells in response to a BCG infection. The inhibited expression of ABC-transporters and ACAT1 was associated with the reduction of intracellular free cholesterol, which in turn induced autophagy in macrophages upon to the Mycobacterial infection. These results strongly suggest an involvement of ABC-transporters and ACAT1 in intracellular cholesterol-mediated autophagy in AMs in response to BCG infection. Conclusion This study thus provides an insight into into a mechanism by which the cholesterol metabolism regulated the autophagy in macrophages in response to mycobacterial infections.http://link.springer.com/article/10.1186/s12865-020-00356-xMycobacterium tuberculosisBovineMacrophageCholesterol metabolismAutophagy |
| spellingShingle | Jinrui Xu Yanbing Zhou Yi Yang Cuiping Lv Xiaoming Liu Yujiong Wang Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection BMC Immunology Mycobacterium tuberculosis Bovine Macrophage Cholesterol metabolism Autophagy |
| title | Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection |
| title_full | Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection |
| title_fullStr | Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection |
| title_full_unstemmed | Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection |
| title_short | Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection |
| title_sort | involvement of abc transporters and acyltransferase 1 in intracellular cholesterol mediated autophagy in bovine alveolar macrophages in response to the bacillus calmette guerin bcg infection |
| topic | Mycobacterium tuberculosis Bovine Macrophage Cholesterol metabolism Autophagy |
| url | http://link.springer.com/article/10.1186/s12865-020-00356-x |
| work_keys_str_mv | AT jinruixu involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection AT yanbingzhou involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection AT yiyang involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection AT cuipinglv involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection AT xiaomingliu involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection AT yujiongwang involvementofabctransportersandacyltransferase1inintracellularcholesterolmediatedautophagyinbovinealveolarmacrophagesinresponsetothebacilluscalmetteguerinbcginfection |