Optimization of extraction-free protocols for SARS-CoV-2 detection using a commercial rRT-PCR assay

Abstract In the ongoing global fight against coronavirus disease 2019 (COVID-19), the sample preparation process for real-time reverse transcription polymerase chain reaction (rRT-PCR) faces challenges due to time-consuming steps, labor-intensive procedures, contamination risks, resource demands, an...

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Bibliographic Details
Main Authors: Minhee Kang, Eunjung Jeong, Ji-Yeon Kim, Sun Ae Yun, Mi-Ae Jang, Ja-Hyun Jang, Tae Yeul Kim, Hee Jae Huh, Nam Yong Lee
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-47645-0