DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity
Apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox factor-1 (Ref-1), is a multifunctional protein that exists widely in living organisms. It can specifically recognize and cleave the DNA in apurinic/apyrimidinic (AP) sites in the base excision repair (BER) pathway, as well as regulate...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Chemistry |
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| Online Access: | https://www.mdpi.com/2624-8549/5/3/124 |
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| author | Hui He Xiaojun Liu Yuchen Wu Lanlin Qi Jin Huang Yan Zhou Jiahao Zeng Kemin Wang Xiaoxiao He |
| author_facet | Hui He Xiaojun Liu Yuchen Wu Lanlin Qi Jin Huang Yan Zhou Jiahao Zeng Kemin Wang Xiaoxiao He |
| author_sort | Hui He |
| collection | DOAJ |
| description | Apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox factor-1 (Ref-1), is a multifunctional protein that exists widely in living organisms. It can specifically recognize and cleave the DNA in apurinic/apyrimidinic (AP) sites in the base excision repair (BER) pathway, as well as regulate the expression of genes to activate some transcription factors. The abnormal expression and disruptions in the biological functions of APE1 are linked to a number of diseases, including inflammation, immunodeficiency, and cancer. Hence, it is extremely desired to monitor the activity of APE1, acquiring a thorough understanding of the healing process of damaged DNA and making clinical diagnoses. Thanks to the advent of DNA nanotechnology, some nanodevices are used to image the activity of APE1 with great sensitivity and simplicity. In this review, we will summarize developments in DNA-nanotechnology-empowered fluorescence imaging in recent years for APE1 activity according to different types of DNA probes, which are classified into linear DNA probes, composite DNA nanomaterials, and three-dimensional (3D) DNA nanostructures. We also highlight the future research directions in the field of APE1 activity imaging. |
| first_indexed | 2024-03-10T22:55:15Z |
| format | Article |
| id | doaj.art-60a9965b68b04e49b6ebbb8f5bae8470 |
| institution | Directory Open Access Journal |
| issn | 2624-8549 |
| language | English |
| last_indexed | 2024-03-10T22:55:15Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemistry |
| spelling | doaj.art-60a9965b68b04e49b6ebbb8f5bae84702023-11-19T10:01:32ZengMDPI AGChemistry2624-85492023-08-01531815183110.3390/chemistry5030124DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 ActivityHui He0Xiaojun Liu1Yuchen Wu2Lanlin Qi3Jin Huang4Yan Zhou5Jiahao Zeng6Kemin Wang7Xiaoxiao He8State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaCollege of Agricultural, Guangxi University, Nanning 530004, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, ChinaApurinic/apyrimidinic endonuclease 1 (APE1), also known as redox factor-1 (Ref-1), is a multifunctional protein that exists widely in living organisms. It can specifically recognize and cleave the DNA in apurinic/apyrimidinic (AP) sites in the base excision repair (BER) pathway, as well as regulate the expression of genes to activate some transcription factors. The abnormal expression and disruptions in the biological functions of APE1 are linked to a number of diseases, including inflammation, immunodeficiency, and cancer. Hence, it is extremely desired to monitor the activity of APE1, acquiring a thorough understanding of the healing process of damaged DNA and making clinical diagnoses. Thanks to the advent of DNA nanotechnology, some nanodevices are used to image the activity of APE1 with great sensitivity and simplicity. In this review, we will summarize developments in DNA-nanotechnology-empowered fluorescence imaging in recent years for APE1 activity according to different types of DNA probes, which are classified into linear DNA probes, composite DNA nanomaterials, and three-dimensional (3D) DNA nanostructures. We also highlight the future research directions in the field of APE1 activity imaging.https://www.mdpi.com/2624-8549/5/3/124APE1DNA nanotechnologyfluorescence imaging |
| spellingShingle | Hui He Xiaojun Liu Yuchen Wu Lanlin Qi Jin Huang Yan Zhou Jiahao Zeng Kemin Wang Xiaoxiao He DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity Chemistry APE1 DNA nanotechnology fluorescence imaging |
| title | DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity |
| title_full | DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity |
| title_fullStr | DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity |
| title_full_unstemmed | DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity |
| title_short | DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity |
| title_sort | dna nanotechnology empowered fluorescence imaging of ape1 activity |
| topic | APE1 DNA nanotechnology fluorescence imaging |
| url | https://www.mdpi.com/2624-8549/5/3/124 |
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