Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo
Human skin contains specialized neuroendocrine Merkel cells responsible for fine touch sensation. In the present study, we performed in-depth analysis of Merkel cells in human fetal back skin. We revealed that these Merkel cells expressed cytokeratin 20 (CK20), were positive for the neuroendocrine m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.983870/full |
| _version_ | 1798033842705006592 |
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| author | Katarzyna Michalak-Micka Katarzyna Michalak-Micka Dominic Rütsche Dominic Rütsche Luca Mazzone Luca Mazzone Luca Mazzone Vanessa L. Büchler Vanessa L. Büchler Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Agnes S. Klar Agnes S. Klar Thomas Biedermann Thomas Biedermann Thomas Biedermann |
| author_facet | Katarzyna Michalak-Micka Katarzyna Michalak-Micka Dominic Rütsche Dominic Rütsche Luca Mazzone Luca Mazzone Luca Mazzone Vanessa L. Büchler Vanessa L. Büchler Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Agnes S. Klar Agnes S. Klar Thomas Biedermann Thomas Biedermann Thomas Biedermann |
| author_sort | Katarzyna Michalak-Micka |
| collection | DOAJ |
| description | Human skin contains specialized neuroendocrine Merkel cells responsible for fine touch sensation. In the present study, we performed in-depth analysis of Merkel cells in human fetal back skin. We revealed that these Merkel cells expressed cytokeratin 20 (CK20), were positive for the neuroendocrine markers synaptophysin and chromogranin A, and the mechanosensitive ion channel Piezo2. Further, we demonstrated that Merkel cells were present in freshly isolated human fetal epidermal cells in vitro, and in tissue-engineered human dermo-epidermal skin substitutes 4 weeks after transplantation on immune-compromised rats. Merkel cells retained the expression of CK20, synaptophysin, chromogranin A, and Piezo2 after isolation and in culture, and in the skin substitutes after transplantation. Interestingly, we observed that in fetal skin and in skin substitutes, only Merkel cells were positive for CK8, while in culture, also non-Merkel cells showed positivity for CK8. In summary, human fetal Merkel cells showed phenotypical features confirming their cell identity. This findings are of pivotal importance for the future application of fetal tissue-engineered skin in clinics. |
| first_indexed | 2024-04-11T20:36:05Z |
| format | Article |
| id | doaj.art-60bf9fb1a7994e4a9e025c38298941bb |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T20:36:05Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-60bf9fb1a7994e4a9e025c38298941bb2022-12-22T04:04:22ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-09-011010.3389/fbioe.2022.983870983870Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivoKatarzyna Michalak-Micka0Katarzyna Michalak-Micka1Dominic Rütsche2Dominic Rütsche3Luca Mazzone4Luca Mazzone5Luca Mazzone6Vanessa L. Büchler7Vanessa L. Büchler8Ueli Moehrlen9Ueli Moehrlen10Ueli Moehrlen11Ueli Moehrlen12Agnes S. Klar13Agnes S. Klar14Thomas Biedermann15Thomas Biedermann16Thomas Biedermann17Tissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandTissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandSpina Bifida Center, University Children’s Hospital Zurich, Zurich, SwitzerlandThe Zurich Center for Fetal Diagnosis and Therapy, University of Zurich, Zurich, SwitzerlandTissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandSpina Bifida Center, University Children’s Hospital Zurich, Zurich, SwitzerlandThe Zurich Center for Fetal Diagnosis and Therapy, University of Zurich, Zurich, SwitzerlandFaculty of Medicine, University of Zurich, Zurich, SwitzerlandTissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandTissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, Zurich, SwitzerlandChildren’s Research Center (CRC), University Children’s Hospital Zurich, Zurich, SwitzerlandFaculty of Medicine, University of Zurich, Zurich, SwitzerlandHuman skin contains specialized neuroendocrine Merkel cells responsible for fine touch sensation. In the present study, we performed in-depth analysis of Merkel cells in human fetal back skin. We revealed that these Merkel cells expressed cytokeratin 20 (CK20), were positive for the neuroendocrine markers synaptophysin and chromogranin A, and the mechanosensitive ion channel Piezo2. Further, we demonstrated that Merkel cells were present in freshly isolated human fetal epidermal cells in vitro, and in tissue-engineered human dermo-epidermal skin substitutes 4 weeks after transplantation on immune-compromised rats. Merkel cells retained the expression of CK20, synaptophysin, chromogranin A, and Piezo2 after isolation and in culture, and in the skin substitutes after transplantation. Interestingly, we observed that in fetal skin and in skin substitutes, only Merkel cells were positive for CK8, while in culture, also non-Merkel cells showed positivity for CK8. In summary, human fetal Merkel cells showed phenotypical features confirming their cell identity. This findings are of pivotal importance for the future application of fetal tissue-engineered skin in clinics.https://www.frontiersin.org/articles/10.3389/fbioe.2022.983870/fullhuman fetal skinMerkel cellCK20skin substituteskin tissue engineering |
| spellingShingle | Katarzyna Michalak-Micka Katarzyna Michalak-Micka Dominic Rütsche Dominic Rütsche Luca Mazzone Luca Mazzone Luca Mazzone Vanessa L. Büchler Vanessa L. Büchler Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Ueli Moehrlen Agnes S. Klar Agnes S. Klar Thomas Biedermann Thomas Biedermann Thomas Biedermann Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo Frontiers in Bioengineering and Biotechnology human fetal skin Merkel cell CK20 skin substitute skin tissue engineering |
| title | Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo |
| title_full | Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo |
| title_fullStr | Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo |
| title_full_unstemmed | Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo |
| title_short | Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo |
| title_sort | human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio engineered skin substitutes in vivo |
| topic | human fetal skin Merkel cell CK20 skin substitute skin tissue engineering |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.983870/full |
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