Imaging single-channel calcium microdomains by total internal reflection microscopy
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electro...
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| Format: | Article |
| Language: | English |
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BMC
2004-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025 |
| _version_ | 1819261842455789568 |
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| author | ANGELO DEMURO IAN PARKER |
| author_facet | ANGELO DEMURO IAN PARKER |
| author_sort | ANGELO DEMURO |
| collection | DOAJ |
| description | The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes |
| first_indexed | 2024-12-23T19:48:13Z |
| format | Article |
| id | doaj.art-60ce77ea5c22409caf5a6e45c35a94f8 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-23T19:48:13Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-60ce77ea5c22409caf5a6e45c35a94f82022-12-21T17:33:27ZengBMCBiological Research0716-97600717-62872004-01-01374675679Imaging single-channel calcium microdomains by total internal reflection microscopyANGELO DEMUROIAN PARKERThe microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocyteshttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025single-channel recordingcalcium imagingTIRFMN-type Ca2+ channels |
| spellingShingle | ANGELO DEMURO IAN PARKER Imaging single-channel calcium microdomains by total internal reflection microscopy Biological Research single-channel recording calcium imaging TIRFM N-type Ca2+ channels |
| title | Imaging single-channel calcium microdomains by total internal reflection microscopy |
| title_full | Imaging single-channel calcium microdomains by total internal reflection microscopy |
| title_fullStr | Imaging single-channel calcium microdomains by total internal reflection microscopy |
| title_full_unstemmed | Imaging single-channel calcium microdomains by total internal reflection microscopy |
| title_short | Imaging single-channel calcium microdomains by total internal reflection microscopy |
| title_sort | imaging single channel calcium microdomains by total internal reflection microscopy |
| topic | single-channel recording calcium imaging TIRFM N-type Ca2+ channels |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025 |
| work_keys_str_mv | AT angelodemuro imagingsinglechannelcalciummicrodomainsbytotalinternalreflectionmicroscopy AT ianparker imagingsinglechannelcalciummicrodomainsbytotalinternalreflectionmicroscopy |