A live-imaging protocol for tracking receptor dynamics in single cells

Summary: Adjacent membrane receptors can show different cellular responses to ligand stimulation. Here, we describe a super-resolution microscopy imaging protocol for tracking the dynamics of two different membrane-bound receptors in single cells. We describe the transfection protocol by electropora...

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Bibliographic Details
Main Authors: Yibin Huang, Toshimasa Takahashi, Herbert Gaisano, Hiromi Rakugi, Koichi Yamamoto
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002271