Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage
The objective of this research was to investigate the effect of astaxanthin supplementations of semen extender on the quality of Hu ram semen after up to five days of preservation at 4 °C. Semen samples were collected from five healthy Hu rams using an artificial vagina during breeding season (April...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/2/319 |
| _version_ | 1797344747958829056 |
|---|---|
| author | Tariq Sohail Liuming Zhang Xuyang Wang Caiyu Jiang Jian Wang Xiaomei Sun Yongjun Li |
| author_facet | Tariq Sohail Liuming Zhang Xuyang Wang Caiyu Jiang Jian Wang Xiaomei Sun Yongjun Li |
| author_sort | Tariq Sohail |
| collection | DOAJ |
| description | The objective of this research was to investigate the effect of astaxanthin supplementations of semen extender on the quality of Hu ram semen after up to five days of preservation at 4 °C. Semen samples were collected from five healthy Hu rams using an artificial vagina during breeding season (April to August 2023) and diluted with a basic extender supplemented with control (0), 1 µM, 2 µM, 3.5 µM, or 4.5 µM of AXT. Overall, 170 semen ejaculate samples (34 repetitions) from five healthy Hu rams were used in our research study. The results revealed that the addition of AXT (3.5 µM) significantly (<i>p</i> ≤ 0.05) increased the sperm kinematic indexes (T.M%, P.M%, MAD%, STR%, and LIN %), sperm viability, plasma membrane integrity, acrosome integrity, total antioxidant content (T-AOC), and mitochondrial membrane potential (MMP) of the Hu rams spermatozoa after up to five days of preservation at 4 °C. Contrary to that, the addition of the best concentration of AXT (3.5 µM) to the semen extender significantly (<i>p</i> ≤ 0.05) reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) concentration of Hu ram semen. In conclusion, the results of the current study indicate that the addition of a semen extender with AXT improves the quality of Hu ram spermatozoa by increasing the total antioxidant capacity (T-AOC) and mitochondrial membrane potential (MMP). On the other hand, reducing free radicals induced oxidative (ROS) and per oxidative (MDA) damage to Hu ram semen. |
| first_indexed | 2024-03-08T11:07:14Z |
| format | Article |
| id | doaj.art-60eaa4b6b8f541d99a80a88150194a72 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:07:14Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-60eaa4b6b8f541d99a80a88150194a722024-01-26T14:34:30ZengMDPI AGAnimals2076-26152024-01-0114231910.3390/ani14020319Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative DamageTariq Sohail0Liuming Zhang1Xuyang Wang2Caiyu Jiang3Jian Wang4Xiaomei Sun5Yongjun Li6Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe objective of this research was to investigate the effect of astaxanthin supplementations of semen extender on the quality of Hu ram semen after up to five days of preservation at 4 °C. Semen samples were collected from five healthy Hu rams using an artificial vagina during breeding season (April to August 2023) and diluted with a basic extender supplemented with control (0), 1 µM, 2 µM, 3.5 µM, or 4.5 µM of AXT. Overall, 170 semen ejaculate samples (34 repetitions) from five healthy Hu rams were used in our research study. The results revealed that the addition of AXT (3.5 µM) significantly (<i>p</i> ≤ 0.05) increased the sperm kinematic indexes (T.M%, P.M%, MAD%, STR%, and LIN %), sperm viability, plasma membrane integrity, acrosome integrity, total antioxidant content (T-AOC), and mitochondrial membrane potential (MMP) of the Hu rams spermatozoa after up to five days of preservation at 4 °C. Contrary to that, the addition of the best concentration of AXT (3.5 µM) to the semen extender significantly (<i>p</i> ≤ 0.05) reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) concentration of Hu ram semen. In conclusion, the results of the current study indicate that the addition of a semen extender with AXT improves the quality of Hu ram spermatozoa by increasing the total antioxidant capacity (T-AOC) and mitochondrial membrane potential (MMP). On the other hand, reducing free radicals induced oxidative (ROS) and per oxidative (MDA) damage to Hu ram semen.https://www.mdpi.com/2076-2615/14/2/319Hu ram semen4 °C preservationastaxanthinsperm qualityantioxidant capacity |
| spellingShingle | Tariq Sohail Liuming Zhang Xuyang Wang Caiyu Jiang Jian Wang Xiaomei Sun Yongjun Li Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage Animals Hu ram semen 4 °C preservation astaxanthin sperm quality antioxidant capacity |
| title | Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage |
| title_full | Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage |
| title_fullStr | Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage |
| title_full_unstemmed | Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage |
| title_short | Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage |
| title_sort | astaxanthin improved the quality of hu ram semen by increasing the antioxidant capacity and mitochondrial potential and mitigating free radicals induced oxidative damage |
| topic | Hu ram semen 4 °C preservation astaxanthin sperm quality antioxidant capacity |
| url | https://www.mdpi.com/2076-2615/14/2/319 |
| work_keys_str_mv | AT tariqsohail astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT liumingzhang astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT xuyangwang astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT caiyujiang astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT jianwang astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT xiaomeisun astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage AT yongjunli astaxanthinimprovedthequalityofhuramsemenbyincreasingtheantioxidantcapacityandmitochondrialpotentialandmitigatingfreeradicalsinducedoxidativedamage |