Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).

Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).

Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulati...

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Bibliographic Details
Main Authors: Thaís Barros Rodrigues, Chitvan Khajuria, Haichuan Wang, Natalie Matz, Danielle Cunha Cardoso, Fernando Hercos Valicente, Xuguo Zhou, Blair Siegfried
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4214676?pdf=render

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http://europepmc.org/articles/PMC4214676?pdf=render

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