Staining and Embedding of Human Chromosomes for 3-D Serial Block-Face Scanning Electron Microscopy

The high-order structure of human chromosomes is an important biological question that is still under investigation. Studies have been done on imaging human mitotic chromosomes using mostly 2-D microscopy methods. To image micron-sized human chromosomes in 3-D, we developed a procedure for preparing...

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Bibliographic Details
Main Authors: Mohammed Yusuf, Bo Chen, Teruo Hashimoto, Ana Katrina Estandarte, George Thompson, Ian Robinson
Format: Article
Language:English
Published: Taylor & Francis Group 2014-12-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114236