A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins.

Bibliographic Details
Main Authors: Anupriya M. Geethakumari, Wesam S. Ahmed, Saad Rasool, Asma Fatima, S. M. Nasir Uddin, Mustapha Aouida, Kabir H. Biswas
Format: Article
Language:English
Published: Nature Portfolio 2022-09-01
Series:Communications Chemistry
Online Access:https://doi.org/10.1038/s42004-022-00731-2

Internet

https://doi.org/10.1038/s42004-022-00731-2

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