A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor
The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-09-01
|
| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-022-00731-2 |
| _version_ | 1811253633836122112 |
|---|---|
| author | Anupriya M. Geethakumari Wesam S. Ahmed Saad Rasool Asma Fatima S. M. Nasir Uddin Mustapha Aouida Kabir H. Biswas |
| author_facet | Anupriya M. Geethakumari Wesam S. Ahmed Saad Rasool Asma Fatima S. M. Nasir Uddin Mustapha Aouida Kabir H. Biswas |
| author_sort | Anupriya M. Geethakumari |
| collection | DOAJ |
| description | The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins. |
| first_indexed | 2024-04-12T16:53:47Z |
| format | Article |
| id | doaj.art-61108c66f7454dde81a54b7b18085740 |
| institution | Directory Open Access Journal |
| issn | 2399-3669 |
| language | English |
| last_indexed | 2024-04-12T16:53:47Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj.art-61108c66f7454dde81a54b7b180857402022-12-22T03:24:18ZengNature PortfolioCommunications Chemistry2399-36692022-09-015111310.1038/s42004-022-00731-2A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensorAnupriya M. Geethakumari0Wesam S. Ahmed1Saad Rasool2Asma Fatima3S. M. Nasir Uddin4Mustapha Aouida5Kabir H. Biswas6Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Genomics and Precision Medicine, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationDivision of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar FoundationThe main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins.https://doi.org/10.1038/s42004-022-00731-2 |
| spellingShingle | Anupriya M. Geethakumari Wesam S. Ahmed Saad Rasool Asma Fatima S. M. Nasir Uddin Mustapha Aouida Kabir H. Biswas A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor Communications Chemistry |
| title | A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor |
| title_full | A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor |
| title_fullStr | A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor |
| title_full_unstemmed | A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor |
| title_short | A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor |
| title_sort | genetically encoded bret based sars cov 2 mpro protease activity sensor |
| url | https://doi.org/10.1038/s42004-022-00731-2 |
| work_keys_str_mv | AT anupriyamgeethakumari ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT wesamsahmed ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT saadrasool ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT asmafatima ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT smnasiruddin ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT mustaphaaouida ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT kabirhbiswas ageneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT anupriyamgeethakumari geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT wesamsahmed geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT saadrasool geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT asmafatima geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT smnasiruddin geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT mustaphaaouida geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor AT kabirhbiswas geneticallyencodedbretbasedsarscov2mproproteaseactivitysensor |