PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet
Objective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract (RPE) treatment. Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal, obesity, obesity treated with ellagic acid (O-EA) and obesity treated with RPE...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-10-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S222116911500129X |
| _version_ | 1830415992038096896 |
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| author | Sri Rahayu Lestari Muhammad Sasmito Djati Achmad Rudijanto Fatchiyah Fatchiyah |
| author_facet | Sri Rahayu Lestari Muhammad Sasmito Djati Achmad Rudijanto Fatchiyah Fatchiyah |
| author_sort | Sri Rahayu Lestari |
| collection | DOAJ |
| description | Objective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract (RPE) treatment.
Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal, obesity, obesity treated with ellagic acid (O-EA) and obesity treated with RPE30 (O-RPE30). Physiological characteristics were monitored by measuring body weight, calorie intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding proteins α and fatty acid binding protein 4 (FABP4) expression were observed using immunohistochemistry, Western blotting and quantitative RT-PCR methods.
Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group and size of adipocyte cells were smaller than obesity group (P < 0.05), but when we compared to normal group, those groups had higher body weight gain and larger adipocyte cells. The level of triglycerides, protein expression of PPARγ and mRNA level of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to obesity group (P < 0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of size of adipocyte, level of triglycerides, PPARγ expression and mRNA level of FABP4 gene on obesity rat model.
Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining size of adipocyte, decreasing triglyceride, PPARγ expression and mRNA level of FABP4 gene on obesity rat model. |
| first_indexed | 2024-12-20T21:16:16Z |
| format | Article |
| id | doaj.art-6119925cb58140b4addcc999050a5085 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-20T21:16:16Z |
| publishDate | 2015-10-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-6119925cb58140b4addcc999050a50852022-12-21T19:26:25ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-10-0151085285710.1016/j.apjtb.2015.01.030PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie dietSri Rahayu Lestari0Muhammad Sasmito Djati1Achmad Rudijanto2Fatchiyah Fatchiyah3Doctoral Program of Biology Department, Faculty of Mathematic and Natural Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, IndonesiaEndocrinology Department, Saiful Anwar Hospital, Jl. Jaksa Agung Suprapto, Malang 65145, East Java, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, IndonesiaObjective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract (RPE) treatment. Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal, obesity, obesity treated with ellagic acid (O-EA) and obesity treated with RPE30 (O-RPE30). Physiological characteristics were monitored by measuring body weight, calorie intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding proteins α and fatty acid binding protein 4 (FABP4) expression were observed using immunohistochemistry, Western blotting and quantitative RT-PCR methods. Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group and size of adipocyte cells were smaller than obesity group (P < 0.05), but when we compared to normal group, those groups had higher body weight gain and larger adipocyte cells. The level of triglycerides, protein expression of PPARγ and mRNA level of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to obesity group (P < 0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of size of adipocyte, level of triglycerides, PPARγ expression and mRNA level of FABP4 gene on obesity rat model. Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining size of adipocyte, decreasing triglyceride, PPARγ expression and mRNA level of FABP4 gene on obesity rat model.http://www.sciencedirect.com/science/article/pii/S222116911500129XHigh calorie dietObesityPeroxisome proliferator activated receptor γRambutan peel extract |
| spellingShingle | Sri Rahayu Lestari Muhammad Sasmito Djati Achmad Rudijanto Fatchiyah Fatchiyah PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet Asian Pacific Journal of Tropical Biomedicine High calorie diet Obesity Peroxisome proliferator activated receptor γ Rambutan peel extract |
| title | PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet |
| title_full | PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet |
| title_fullStr | PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet |
| title_full_unstemmed | PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet |
| title_short | PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet |
| title_sort | pparγ expression by rambutan peel extract in obesity rat model induced high calorie diet |
| topic | High calorie diet Obesity Peroxisome proliferator activated receptor γ Rambutan peel extract |
| url | http://www.sciencedirect.com/science/article/pii/S222116911500129X |
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