Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice
Abstract Background Alzheimer's disease is accompanied by an abnormal high accumulation of cis-P tau. However, the long-term changes in behavior following tau accumulation remains under debate. The present study investigated the long-term effects of tauopathy on learning and memory, synaptic pl...
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| Format: | Article |
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BMC
2023-05-01
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| Series: | Behavioral and Brain Functions |
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| Online Access: | https://doi.org/10.1186/s12993-023-00211-0 |
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| author | Bakhtiarzadeh Fatemeh Shahpasand Koorosh Shojaei Amir Fathollahi Yaghoub Mirnajafi-Zadeh Javad |
| author_facet | Bakhtiarzadeh Fatemeh Shahpasand Koorosh Shojaei Amir Fathollahi Yaghoub Mirnajafi-Zadeh Javad |
| author_sort | Bakhtiarzadeh Fatemeh |
| collection | DOAJ |
| description | Abstract Background Alzheimer's disease is accompanied by an abnormal high accumulation of cis-P tau. However, the long-term changes in behavior following tau accumulation remains under debate. The present study investigated the long-term effects of tauopathy on learning and memory, synaptic plasticity, and hippocampal cell numbers. Results Cis-P tau was microinjected into the dorsal hippocampus to generate Alzheimer’s like-disease model in C57BL/6 mice. Cis-P tau injected animals showed a significant impairment in learning and memory in Y-maze and Barnes maze tests. In another group of animals, the generation of long-term potentiation (LTP) was evaluated in hippocampal slices 7 months after cis-P tau injection. LTP induction was disrupted only in the dorsal but not ventral hippocampal slices. The basal synaptic transmission was also reduced in dorsal hippocampal slices. In addition, hippocampal sampling was done, and the number of cells was assessed by Nissl staining. Obtained results indicated that the number of survived cells was significantly reduced in the dorsal and ventral hippocampus of cis P-tau injected animals compared to the animals in control group. However, the decrement of cell number was higher in the dorsal compared to the ventral hippocampus. Conclusions In conclusion, intra-hippocampal cis-P tau injection produced learning and memory impairment at 7 months after its injection. This impairment might result from LTP disruption and a significant decrease in the number of neurons in the dorsal hippocampus. Graphical Abstract |
| first_indexed | 2024-03-13T08:59:20Z |
| format | Article |
| id | doaj.art-61384b579c5b473c8c05d9c19772f9c9 |
| institution | Directory Open Access Journal |
| issn | 1744-9081 |
| language | English |
| last_indexed | 2024-03-13T08:59:20Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Behavioral and Brain Functions |
| spelling | doaj.art-61384b579c5b473c8c05d9c19772f9c92023-05-28T11:23:17ZengBMCBehavioral and Brain Functions1744-90812023-05-0119111310.1186/s12993-023-00211-0Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in miceBakhtiarzadeh Fatemeh0Shahpasand Koorosh1Shojaei Amir2Fathollahi Yaghoub3Mirnajafi-Zadeh Javad4Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECRDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares UniversityAbstract Background Alzheimer's disease is accompanied by an abnormal high accumulation of cis-P tau. However, the long-term changes in behavior following tau accumulation remains under debate. The present study investigated the long-term effects of tauopathy on learning and memory, synaptic plasticity, and hippocampal cell numbers. Results Cis-P tau was microinjected into the dorsal hippocampus to generate Alzheimer’s like-disease model in C57BL/6 mice. Cis-P tau injected animals showed a significant impairment in learning and memory in Y-maze and Barnes maze tests. In another group of animals, the generation of long-term potentiation (LTP) was evaluated in hippocampal slices 7 months after cis-P tau injection. LTP induction was disrupted only in the dorsal but not ventral hippocampal slices. The basal synaptic transmission was also reduced in dorsal hippocampal slices. In addition, hippocampal sampling was done, and the number of cells was assessed by Nissl staining. Obtained results indicated that the number of survived cells was significantly reduced in the dorsal and ventral hippocampus of cis P-tau injected animals compared to the animals in control group. However, the decrement of cell number was higher in the dorsal compared to the ventral hippocampus. Conclusions In conclusion, intra-hippocampal cis-P tau injection produced learning and memory impairment at 7 months after its injection. This impairment might result from LTP disruption and a significant decrease in the number of neurons in the dorsal hippocampus. Graphical Abstracthttps://doi.org/10.1186/s12993-023-00211-0Alzheimer’s like diseaseCis P-tauDorsal and ventral hippocampusLearning and memory and synaptic plasticity |
| spellingShingle | Bakhtiarzadeh Fatemeh Shahpasand Koorosh Shojaei Amir Fathollahi Yaghoub Mirnajafi-Zadeh Javad Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice Behavioral and Brain Functions Alzheimer’s like disease Cis P-tau Dorsal and ventral hippocampus Learning and memory and synaptic plasticity |
| title | Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice |
| title_full | Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice |
| title_fullStr | Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice |
| title_full_unstemmed | Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice |
| title_short | Intra-hippocampal cis-P tau microinjection induces long-term changes in behavior and synaptic plasticity in mice |
| title_sort | intra hippocampal cis p tau microinjection induces long term changes in behavior and synaptic plasticity in mice |
| topic | Alzheimer’s like disease Cis P-tau Dorsal and ventral hippocampus Learning and memory and synaptic plasticity |
| url | https://doi.org/10.1186/s12993-023-00211-0 |
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