Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

Summary: The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using s...

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Bibliographic Details
Main Authors: Adele Moatti, Yuheng Cai, Chen Li, Kristen D. Popowski, Ke Cheng, Frances S. Ligler, Alon Greenbaum
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001788