Assessment of <italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Silico</italic> Protocols for Sequence-Based Characterization of the Human Vaginal Microbiome

Assessment of <italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Silico</italic> Protocols for Sequence-Based Characterization of the Human Vaginal Microbiome

ABSTRACT The vaginal microbiome has been connected to a wide range of health outcomes. This has led to a thriving research environment but also to the use of conflicting methodologies to study its microbial composition. Here, we systematically assessed best practices for the sequencing-based charact...

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Bibliographic Details
Main Authors: Luisa W. Hugerth, Marcela Pereira, Yinghua Zha, Maike Seifert, Vilde Kaldhusdal, Fredrik Boulund, Maria C. Krog, Zahra Bashir, Marica Hamsten, Emma Fransson, Henriette Svarre Nielsen, Ina Schuppe-Koistinen, Lars Engstrand
Format: Article
Language:English
Published: American Society for Microbiology 2020-12-01
Series:mSphere
Subjects:
16S rRNA
PCR
amplicon
human microbiome
metagenomics
molecular methods
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00448-20

Internet

https://journals.asm.org/doi/10.1128/mSphere.00448-20

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