Detection of SNARE complexes with FRET using the tetracysteine system

The three proteins synaptosomal-associated protein 25 (SNAP-25), Syntaxin-1a and vesicle-associated membrane protein (VAMP-2) are collectively called SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors). By assembling into an exocytic complex, the three SNAREs help in cata...

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Bibliographic Details
Main Author: Oleg Varlamov
Format: Article
Language:English
Published: Taylor & Francis Group 2012-02-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000113811