Optimized Protocols for In-Vitro T-Cell-Dependent and T-Cell-Independent Activation for B-Cell Differentiation Studies Using Limited Cells

Optimized Protocols for In-Vitro T-Cell-Dependent and T-Cell-Independent Activation for B-Cell Differentiation Studies Using Limited Cells

Background/MethodsFor mechanistic studies, in-vitro human B-cell differentiation and generation of plasma cells are invaluable techniques. However, the heterogeneity of both T-cell-dependent (TD) and T-cell-independent (TI) stimuli and the disparity of culture conditions used in existing protocols m...

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Bibliographic Details
Main Authors: Casper Marsman, Dorit Verhoeven, Jana Koers, Theo Rispens, Anja ten Brinke, S. Marieke van Ham, Taco W. Kuijpers
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Immunology
Subjects:
B-cell activation
B-cell differentiation
plasma cells
CD40L
IL-21
CpG
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2022.815449/full

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https://www.frontiersin.org/articles/10.3389/fimmu.2022.815449/full

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