Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study
Osteochondral grafts are used for repair of focal osteochondral lesions. Autologous grafts are the gold standard treatment; however, limited graft availability and donor site morbidity restrict use. Therefore, there is a clinical need for different graft sources/materials which replicate natural car...
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| Format: | Article |
| Language: | English |
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The Royal Society
2024-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.230431 |
| _version_ | 1797360180714799104 |
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| author | Raelene M. Cowie Laura Macri-Pellizzeri Jane McLaren William J. Sanderson Reda M. Felfel Colin A. Scotchford Brigitte E. Scammell David M. Grant Virginie Sottile Louise M. Jennings |
| author_facet | Raelene M. Cowie Laura Macri-Pellizzeri Jane McLaren William J. Sanderson Reda M. Felfel Colin A. Scotchford Brigitte E. Scammell David M. Grant Virginie Sottile Louise M. Jennings |
| author_sort | Raelene M. Cowie |
| collection | DOAJ |
| description | Osteochondral grafts are used for repair of focal osteochondral lesions. Autologous grafts are the gold standard treatment; however, limited graft availability and donor site morbidity restrict use. Therefore, there is a clinical need for different graft sources/materials which replicate natural cartilage function. Chitosan has been proposed for this application. The aim of this study was to assess the biomechanics and biotribology of a bioresorbable chitosan/chitosan-nano-hydroxyapatite osteochondral construct (OCC), implanted in an in vitro porcine knee experimental simulation model. The OCC implanted in different surgical positions (flush, proud and inverted) was compared to predicate grafts in current clinical use and a positive control consisting of a stainless steel graft implanted proud of the cartilage surface. After 3 h (10 800 cycles) wear simulation under a walking gait, subsidence occurred in all OCC samples irrespective of surgical positioning, but with no apparent loss of material and low meniscus wear. Half the predicate grafts exhibited delamination and scratching of the cartilage surfaces. No graft subsidence occurred in the positive controls but wear and deformation of the meniscus were apparent. Implanting a new chitosan-based OCC either optimally (flush), inverted or proud of the cartilage surface resulted in minimal wear, damage and deformation of the meniscus. |
| first_indexed | 2024-03-08T15:35:35Z |
| format | Article |
| id | doaj.art-61a17e15969c435facfffdf6411f3e42 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-03-08T15:35:35Z |
| publishDate | 2024-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-61a17e15969c435facfffdf6411f3e422024-01-10T00:05:55ZengThe Royal SocietyRoyal Society Open Science2054-57032024-01-0111110.1098/rsos.230431Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological studyRaelene M. Cowie0Laura Macri-Pellizzeri1Jane McLaren2William J. Sanderson3Reda M. Felfel4Colin A. Scotchford5Brigitte E. Scammell6David M. Grant7Virginie Sottile8Louise M. Jennings9Institute of Medical and Biological Engineering, University of Leeds, Leeds, UKAcademic Unit Translational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UKAcademic Unit Injury, Recovery and Inflammation Sciences (IRIS), School of Medicine, University of Nottingham, Nottingham, UKInstitute of Medical and Biological Engineering, University of Leeds, Leeds, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham, UKAcademic Unit Injury, Recovery and Inflammation Sciences (IRIS), School of Medicine, University of Nottingham, Nottingham, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham, UKAcademic Unit Translational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UKInstitute of Medical and Biological Engineering, University of Leeds, Leeds, UKOsteochondral grafts are used for repair of focal osteochondral lesions. Autologous grafts are the gold standard treatment; however, limited graft availability and donor site morbidity restrict use. Therefore, there is a clinical need for different graft sources/materials which replicate natural cartilage function. Chitosan has been proposed for this application. The aim of this study was to assess the biomechanics and biotribology of a bioresorbable chitosan/chitosan-nano-hydroxyapatite osteochondral construct (OCC), implanted in an in vitro porcine knee experimental simulation model. The OCC implanted in different surgical positions (flush, proud and inverted) was compared to predicate grafts in current clinical use and a positive control consisting of a stainless steel graft implanted proud of the cartilage surface. After 3 h (10 800 cycles) wear simulation under a walking gait, subsidence occurred in all OCC samples irrespective of surgical positioning, but with no apparent loss of material and low meniscus wear. Half the predicate grafts exhibited delamination and scratching of the cartilage surfaces. No graft subsidence occurred in the positive controls but wear and deformation of the meniscus were apparent. Implanting a new chitosan-based OCC either optimally (flush), inverted or proud of the cartilage surface resulted in minimal wear, damage and deformation of the meniscus.https://royalsocietypublishing.org/doi/10.1098/rsos.230431chitosantribologyosteochondral graftnatural knee jointjoint simulation |
| spellingShingle | Raelene M. Cowie Laura Macri-Pellizzeri Jane McLaren William J. Sanderson Reda M. Felfel Colin A. Scotchford Brigitte E. Scammell David M. Grant Virginie Sottile Louise M. Jennings Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study Royal Society Open Science chitosan tribology osteochondral graft natural knee joint joint simulation |
| title | Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study |
| title_full | Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study |
| title_fullStr | Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study |
| title_full_unstemmed | Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study |
| title_short | Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study |
| title_sort | functional performance of a bi layered chitosan nano hydroxyapatite osteochondral scaffold a pre clinical in vitro tribological study |
| topic | chitosan tribology osteochondral graft natural knee joint joint simulation |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.230431 |
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