Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Abstract Background The unnatural amino acid, L-2-aminobutyric acid (L-ABA) is an essential chiral building block for various pharmaceutical drugs, such as the antiepileptic drug levetiracetam and the antituberculosis drug ethambutol. The present study aims at obtaining variants of ω-transaminase fr...

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Bibliographic Details
Main Authors: Zhiwei Zhang, Yang Liu, Jing Zhao, Wenqiang Li, Ruiwen Hu, Xia Li, Aitao Li, Yaping Wang, Lixin Ma
Format: Article
Language:English
Published: BMC 2021-09-01
Series:BMC Biotechnology
Subjects:
ω-transaminase
Saturation mutagenesis
L-2-aminobutyric acid
Molecular docking
L57C/M419I variant
Online Access:https://doi.org/10.1186/s12896-021-00713-7

Internet

https://doi.org/10.1186/s12896-021-00713-7

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