Development of isothermal TaqMan assays for detection of biothreat organisms

TaqMan probe (dual-labeled DNA probe)–based real-time detection, one of the most sensitive and specific fluorescent detection methods, has been widely utilized in conjunction with polymerase chain reaction (PCR). Helicase-dependent amplification (HDA) is an isothermal amplification technology that h...

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Bibliographic Details
Main Authors: Yanhong Tong, Wen Tang, Hyun-Jin Kim, Xiaojing Pan, Tamara A. Ranalli, Huimin Kong
Format: Article
Language:English
Published: Taylor & Francis Group 2008-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112959