Development of isothermal TaqMan assays for detection of biothreat organisms
TaqMan probe (dual-labeled DNA probe)–based real-time detection, one of the most sensitive and specific fluorescent detection methods, has been widely utilized in conjunction with polymerase chain reaction (PCR). Helicase-dependent amplification (HDA) is an isothermal amplification technology that h...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2008-11-01
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Series: | BioTechniques |
Online Access: | https://www.future-science.com/doi/10.2144/000112959 |