Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway
Abstract Background To investigate the therapeutic effects of menstrual blood derived mesenchymal stem cells (MB-MSCs) combined with Bushen Tiaochong recipe (BSTCR) on epirubicin induced premature ovarian failure (POF) in mice. Methods Twenty-four female C57BL/6 mice of 6–8 weeks were intraperitonea...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-019-0499-2 |
| _version_ | 1811299145972973568 |
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| author | Fengyi Guo Tian Xia Yedan Zhang Xiaotong Ma Zhongrui Yan Shaohua Hao Yali Han Ruihong Ma Yuan Zhou Xue Du |
| author_facet | Fengyi Guo Tian Xia Yedan Zhang Xiaotong Ma Zhongrui Yan Shaohua Hao Yali Han Ruihong Ma Yuan Zhou Xue Du |
| author_sort | Fengyi Guo |
| collection | DOAJ |
| description | Abstract Background To investigate the therapeutic effects of menstrual blood derived mesenchymal stem cells (MB-MSCs) combined with Bushen Tiaochong recipe (BSTCR) on epirubicin induced premature ovarian failure (POF) in mice. Methods Twenty-four female C57BL/6 mice of 6–8 weeks were intraperitoneally injected with epirubicin to induce POF, and then they were randomized into 4 groups of 6 mice each and treated with PBS, MB-MSCs, BSTCR, and MB-MSCs combined with BSTCR, respectively. Six mice of the same age were used as controls. Vaginal smear, TUNEL and hematoxylin-eosin staining were to observe estrous cycles, ovarian cell apoptosis and follicles. Enzyme-linked immunosorbent analysis determined serum estradiol, follicle-stimulating hormone (FSH) and anti-Müllerian hormone (AMH) levels. RT-qPCR and Western Blot analysis were to determine GADD45b, CyclinB1, CDC2 and pCDC2 expressions. Results Epirubicin treatment resulted in a decrease in the number of primordial, primary, secondary and antral follicles, an increase in the number of atretic follicles and ovarian cell apoptosis, a decrease in estradiol and AMH levels, an increase in FSH levels, and estrous cycle arrest. However, MB-MSCs combined with BSTCR rescued epirubicin induced POF through down-regulating GADD45b and pCDC2 expressions, and up-regulating CyclinB1 and CDC2 expressions. The combined treatment showed better therapeutic efficacy than BSTCR or MB-MSCs alone. Conclusions MB-MSCs combined with BSTCR improved the ovarian function of epirubicin induced POF mice, which might be related to the inhibition of GADD45b expression and the promotion of CyclinB1 and CDC2 expressions. The combined treatment had better therapeutic efficacy than BSTCR or MB-MSCs alone. |
| first_indexed | 2024-04-13T06:31:56Z |
| format | Article |
| id | doaj.art-621ea052b5bf431e908fb85118ee4f80 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-13T06:31:56Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-621ea052b5bf431e908fb85118ee4f802022-12-22T02:58:07ZengBMCReproductive Biology and Endocrinology1477-78272019-07-0117111210.1186/s12958-019-0499-2Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathwayFengyi Guo0Tian Xia1Yedan Zhang2Xiaotong Ma3Zhongrui Yan4Shaohua Hao5Yali Han6Ruihong Ma7Yuan Zhou8Xue Du9Department of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityReproductive Center, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityReproductive Center, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineState Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeDepartment of Obstetrics & Gynecology, General Hospital, Tianjin Medical UniversityAbstract Background To investigate the therapeutic effects of menstrual blood derived mesenchymal stem cells (MB-MSCs) combined with Bushen Tiaochong recipe (BSTCR) on epirubicin induced premature ovarian failure (POF) in mice. Methods Twenty-four female C57BL/6 mice of 6–8 weeks were intraperitoneally injected with epirubicin to induce POF, and then they were randomized into 4 groups of 6 mice each and treated with PBS, MB-MSCs, BSTCR, and MB-MSCs combined with BSTCR, respectively. Six mice of the same age were used as controls. Vaginal smear, TUNEL and hematoxylin-eosin staining were to observe estrous cycles, ovarian cell apoptosis and follicles. Enzyme-linked immunosorbent analysis determined serum estradiol, follicle-stimulating hormone (FSH) and anti-Müllerian hormone (AMH) levels. RT-qPCR and Western Blot analysis were to determine GADD45b, CyclinB1, CDC2 and pCDC2 expressions. Results Epirubicin treatment resulted in a decrease in the number of primordial, primary, secondary and antral follicles, an increase in the number of atretic follicles and ovarian cell apoptosis, a decrease in estradiol and AMH levels, an increase in FSH levels, and estrous cycle arrest. However, MB-MSCs combined with BSTCR rescued epirubicin induced POF through down-regulating GADD45b and pCDC2 expressions, and up-regulating CyclinB1 and CDC2 expressions. The combined treatment showed better therapeutic efficacy than BSTCR or MB-MSCs alone. Conclusions MB-MSCs combined with BSTCR improved the ovarian function of epirubicin induced POF mice, which might be related to the inhibition of GADD45b expression and the promotion of CyclinB1 and CDC2 expressions. The combined treatment had better therapeutic efficacy than BSTCR or MB-MSCs alone.http://link.springer.com/article/10.1186/s12958-019-0499-2Mesenchymal stem cellsMenstrual bloodBushen Tiaochong recipePremature ovarian failureEpirubicinGADD45 |
| spellingShingle | Fengyi Guo Tian Xia Yedan Zhang Xiaotong Ma Zhongrui Yan Shaohua Hao Yali Han Ruihong Ma Yuan Zhou Xue Du Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway Reproductive Biology and Endocrinology Mesenchymal stem cells Menstrual blood Bushen Tiaochong recipe Premature ovarian failure Epirubicin GADD45 |
| title | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway |
| title_full | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway |
| title_fullStr | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway |
| title_full_unstemmed | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway |
| title_short | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in mice by inhibiting GADD45b expression in the cell cycle pathway |
| title_sort | menstrual blood derived mesenchymal stem cells combined with bushen tiaochong recipe improved chemotherapy induced premature ovarian failure in mice by inhibiting gadd45b expression in the cell cycle pathway |
| topic | Mesenchymal stem cells Menstrual blood Bushen Tiaochong recipe Premature ovarian failure Epirubicin GADD45 |
| url | http://link.springer.com/article/10.1186/s12958-019-0499-2 |
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