High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

Abstract Background Production of 3-hydroxypropionic acid (3-HP) through the malonyl-CoA pathway has yielded promising results in Pichia pastoris (Komagataella phaffii), demonstrating the potential of this cell factory to produce this platform chemical and other acetyl-CoA-derived products using gly...

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Bibliographic Details
Main Authors: Albert Fina, Pierre Millard, Joan Albiol, Pau Ferrer, Stephanie Heux
Format: Article
Language:English
Published: BMC 2023-06-01
Series:Microbial Cell Factories
Subjects:
Fluxomics
13C-Metabolic flux analysis
Pichia pastoris
Komagataella phaffii
High throughput
3-hydroxypropionic acid
Online Access:https://doi.org/10.1186/s12934-023-02123-0

Internet

https://doi.org/10.1186/s12934-023-02123-0

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