Rapid activation of hematopoietic stem cells
Abstract Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent. Following perturbations, such as blood loss or infection, HSCs may undergo activation. Surprisingly, little is known about the earliest stages of HSCs activation. We utilize surface markers of HSCs activation, CD69...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-023-03377-6 |
| _version_ | 1797806845697458176 |
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| author | Roshina Thapa Erez Elfassy Leonid Olender Omri Sharabi Roi Gazit |
| author_facet | Roshina Thapa Erez Elfassy Leonid Olender Omri Sharabi Roi Gazit |
| author_sort | Roshina Thapa |
| collection | DOAJ |
| description | Abstract Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent. Following perturbations, such as blood loss or infection, HSCs may undergo activation. Surprisingly, little is known about the earliest stages of HSCs activation. We utilize surface markers of HSCs activation, CD69 and CD317, revealing a response as early as 2 h after stimulation. The dynamic expression of HSCs activation markers varies between viral-like (poly-Inosinic-poly-Cytidylic) or bacterial-like (Lipopolysaccharide) immune stimuli. We further quantify dose response, revealing a low threshold, and similar sensitivity of HSCs and progenitors in the BM. Finally, we find a positive correlation between the expression of surface activation markers and early exit from quiescence. Our data show that the response of adult stem cells to immune stimulation is rapid and sensitive, rapidly leading HSCs out of quiescence. |
| first_indexed | 2024-03-13T06:13:33Z |
| format | Article |
| id | doaj.art-6245967585cc4582854220be55eea2b7 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-03-13T06:13:33Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-6245967585cc4582854220be55eea2b72023-06-11T11:07:09ZengBMCStem Cell Research & Therapy1757-65122023-06-0114111010.1186/s13287-023-03377-6Rapid activation of hematopoietic stem cellsRoshina Thapa0Erez Elfassy1Leonid Olender2Omri Sharabi3Roi Gazit4The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the NegevThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the NegevThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the NegevThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the NegevThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the NegevAbstract Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent. Following perturbations, such as blood loss or infection, HSCs may undergo activation. Surprisingly, little is known about the earliest stages of HSCs activation. We utilize surface markers of HSCs activation, CD69 and CD317, revealing a response as early as 2 h after stimulation. The dynamic expression of HSCs activation markers varies between viral-like (poly-Inosinic-poly-Cytidylic) or bacterial-like (Lipopolysaccharide) immune stimuli. We further quantify dose response, revealing a low threshold, and similar sensitivity of HSCs and progenitors in the BM. Finally, we find a positive correlation between the expression of surface activation markers and early exit from quiescence. Our data show that the response of adult stem cells to immune stimulation is rapid and sensitive, rapidly leading HSCs out of quiescence.https://doi.org/10.1186/s13287-023-03377-6Hematopoietic stem cellsHSCActivation markersRapid activationCD69CD317 |
| spellingShingle | Roshina Thapa Erez Elfassy Leonid Olender Omri Sharabi Roi Gazit Rapid activation of hematopoietic stem cells Stem Cell Research & Therapy Hematopoietic stem cells HSC Activation markers Rapid activation CD69 CD317 |
| title | Rapid activation of hematopoietic stem cells |
| title_full | Rapid activation of hematopoietic stem cells |
| title_fullStr | Rapid activation of hematopoietic stem cells |
| title_full_unstemmed | Rapid activation of hematopoietic stem cells |
| title_short | Rapid activation of hematopoietic stem cells |
| title_sort | rapid activation of hematopoietic stem cells |
| topic | Hematopoietic stem cells HSC Activation markers Rapid activation CD69 CD317 |
| url | https://doi.org/10.1186/s13287-023-03377-6 |
| work_keys_str_mv | AT roshinathapa rapidactivationofhematopoieticstemcells AT erezelfassy rapidactivationofhematopoieticstemcells AT leonidolender rapidactivationofhematopoieticstemcells AT omrisharabi rapidactivationofhematopoieticstemcells AT roigazit rapidactivationofhematopoieticstemcells |