Protocol for lentiviral vector-based gene transfection in human ILC2s

Summary: Modifying gene expression in lymphocytes is essential for immunological research; however, gene transfection methods for group 2 innate lymphoid cells (ILC2s) are not well established. Here, we present a protocol utilizing lentiviral vectors to introduce genes into human ILC2s. We detail st...

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Bibliographic Details
Main Authors: Misato Irie, Hiroki Kabata, Koichi Fukunaga
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000194