Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults
Abstract To evaluate clinical value of metagenomic next-generation sequencing (mNGS) in people living with HIV/AIDS (PLWHA) who had CNS disorders. Cerebrospinal fluid (CSF) samples from 48 PLWHA presenting with CNS disorders were sequenced using mNGS and compared with clinical conventional diagnosti...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31974-1 |
| _version_ | 1797859953586733056 |
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| author | Liping Deng Qian Li Wenjia Hu Xiaoping Chen Tielong Chen Shihui Song Pingzheng Mo Shi Zou Yongxi Zhang Shicheng Gao Liangjun Chen Ke Zhuang Rongrong Yang Yong Xiong |
| author_facet | Liping Deng Qian Li Wenjia Hu Xiaoping Chen Tielong Chen Shihui Song Pingzheng Mo Shi Zou Yongxi Zhang Shicheng Gao Liangjun Chen Ke Zhuang Rongrong Yang Yong Xiong |
| author_sort | Liping Deng |
| collection | DOAJ |
| description | Abstract To evaluate clinical value of metagenomic next-generation sequencing (mNGS) in people living with HIV/AIDS (PLWHA) who had CNS disorders. Cerebrospinal fluid (CSF) samples from 48 PLWHA presenting with CNS disorders were sequenced using mNGS and compared with clinical conventional diagnostic methods. In total, 36/48 ss(75%) patients were diagnosed with pathogen(s) infection by mNGS, and the positive detection proportion by mNGS was higher than that by clinical conventional diagnostic methods (75% vs 52.1%, X 2 = 5.441, P = 0.020). Thirteen out of 48 patients (27.1%) were detected with 3–7 pathogens by mNGS. Moreover, 77 pathogen strains were detected, of which 94.8% (73/77) by mNGS and 37.0% (30/77) by clinical conventional methods (X 2 = 54.206, P < 0.001). The sensitivity and specificity of pathogens detection by mNGS were 63.9% (23/36) and 66.7% (8/12), respectively, which were superior to that by clinical conventional methods (23/36 vs 9/25, X 2 = 4.601, P = 0.032; 8/12 vs 5/23, X 2 = 5.029, P = 0.009). The application of mNGS was superior for its ability to detect a variety of unknown pathogens and multiple pathogens infection, and relatively higher sensitivity and specificity in diagnosis of CNS disorders in PLWHA. |
| first_indexed | 2024-04-09T21:37:55Z |
| format | Article |
| id | doaj.art-62c3f80cbb864d3bb6bdf0aec9bd68c4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T21:37:55Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-62c3f80cbb864d3bb6bdf0aec9bd68c42023-03-26T11:11:26ZengNature PortfolioScientific Reports2045-23222023-03-0113111010.1038/s41598-023-31974-1Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adultsLiping Deng0Qian Li1Wenjia Hu2Xiaoping Chen3Tielong Chen4Shihui Song5Pingzheng Mo6Shi Zou7Yongxi Zhang8Shicheng Gao9Liangjun Chen10Ke Zhuang11Rongrong Yang12Yong Xiong13Department of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Laboratory Medicine, Zhongnan Hospital, Wuhan UniversityABSL‐III Laboratory at the Center for Animal Experiment, State Key Laboratory of Virology, Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityAbstract To evaluate clinical value of metagenomic next-generation sequencing (mNGS) in people living with HIV/AIDS (PLWHA) who had CNS disorders. Cerebrospinal fluid (CSF) samples from 48 PLWHA presenting with CNS disorders were sequenced using mNGS and compared with clinical conventional diagnostic methods. In total, 36/48 ss(75%) patients were diagnosed with pathogen(s) infection by mNGS, and the positive detection proportion by mNGS was higher than that by clinical conventional diagnostic methods (75% vs 52.1%, X 2 = 5.441, P = 0.020). Thirteen out of 48 patients (27.1%) were detected with 3–7 pathogens by mNGS. Moreover, 77 pathogen strains were detected, of which 94.8% (73/77) by mNGS and 37.0% (30/77) by clinical conventional methods (X 2 = 54.206, P < 0.001). The sensitivity and specificity of pathogens detection by mNGS were 63.9% (23/36) and 66.7% (8/12), respectively, which were superior to that by clinical conventional methods (23/36 vs 9/25, X 2 = 4.601, P = 0.032; 8/12 vs 5/23, X 2 = 5.029, P = 0.009). The application of mNGS was superior for its ability to detect a variety of unknown pathogens and multiple pathogens infection, and relatively higher sensitivity and specificity in diagnosis of CNS disorders in PLWHA.https://doi.org/10.1038/s41598-023-31974-1 |
| spellingShingle | Liping Deng Qian Li Wenjia Hu Xiaoping Chen Tielong Chen Shihui Song Pingzheng Mo Shi Zou Yongxi Zhang Shicheng Gao Liangjun Chen Ke Zhuang Rongrong Yang Yong Xiong Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults Scientific Reports |
| title | Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults |
| title_full | Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults |
| title_fullStr | Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults |
| title_full_unstemmed | Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults |
| title_short | Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults |
| title_sort | metagenomic next generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in hiv infected chinese adults |
| url | https://doi.org/10.1038/s41598-023-31974-1 |
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