Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice
Benzo[a]pyrene (BaP), as a highly toxic substance, exists in the surrounding and foods of people's life and poses a great threat to the human body. Our previous studies have shown that BaP caused oxidative and pyroptotic damage to the liver, which is an urgent problem to be solved. Whole grains...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622001529 |
| _version_ | 1818212935241039872 |
|---|---|
| author | Hao Li Li Yuan Zhulin Wang Lin Shi Rui Dong Xinzhong Hu |
| author_facet | Hao Li Li Yuan Zhulin Wang Lin Shi Rui Dong Xinzhong Hu |
| author_sort | Hao Li |
| collection | DOAJ |
| description | Benzo[a]pyrene (BaP), as a highly toxic substance, exists in the surrounding and foods of people's life and poses a great threat to the human body. Our previous studies have shown that BaP caused oxidative and pyroptotic damage to the liver, which is an urgent problem to be solved. Whole grains not only contain a series of important nutrients, but also have important significance for preventing obesity, dyslipidemia and inflammation. Therefore, this work tried to use whole grain diet as a new method to inhibit the BaP-induced toxicity in mice. Compared with BaP group, the addition of whole grain buckwheat (WGB) or oat (WGO) diet could improve the appetite of mice, stabilize the levels of ALT, AST, TC and TG in serum, maintain the balance of H2O2, MDA, GSH-Px and SOD levels in liver tissues, reduce BPDE-DNA adduct content and the mRNA expression of BaP metabolites related enzyme (including CYP1A1, CYP1B1, GST-P1 and AhR). Furthermore, both WGB and WGO could inhibit the pyroptotic markers (NLRP3, Caspase-1, GSDMD) increase and inflammatory factors release, promote the synthesis of Short-chain fatty acids (SCFAs), and the effect of WGO was more excellent than WGB. This study fully verified that the reverse effect of the dietary whole grain on BaP-induced genotoxicity, oxidative and pyroptotic injury in liver of mice, and provided a powerful theoretical support for new toxicity control dietary methods in the future. |
| first_indexed | 2024-12-12T05:56:18Z |
| format | Article |
| id | doaj.art-62d0d0bfed8f49fda6821f543db53315 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-12T05:56:18Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-62d0d0bfed8f49fda6821f543db533152022-12-22T00:35:33ZengElsevierJournal of Functional Foods1756-46462022-06-0193105082Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of miceHao Li0Li Yuan1Zhulin Wang2Lin Shi3Rui Dong4Xinzhong Hu5Engineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaCorresponding authors.; Engineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaEngineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaEngineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaEngineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaCorresponding authors.; Engineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, People’s Republic of ChinaBenzo[a]pyrene (BaP), as a highly toxic substance, exists in the surrounding and foods of people's life and poses a great threat to the human body. Our previous studies have shown that BaP caused oxidative and pyroptotic damage to the liver, which is an urgent problem to be solved. Whole grains not only contain a series of important nutrients, but also have important significance for preventing obesity, dyslipidemia and inflammation. Therefore, this work tried to use whole grain diet as a new method to inhibit the BaP-induced toxicity in mice. Compared with BaP group, the addition of whole grain buckwheat (WGB) or oat (WGO) diet could improve the appetite of mice, stabilize the levels of ALT, AST, TC and TG in serum, maintain the balance of H2O2, MDA, GSH-Px and SOD levels in liver tissues, reduce BPDE-DNA adduct content and the mRNA expression of BaP metabolites related enzyme (including CYP1A1, CYP1B1, GST-P1 and AhR). Furthermore, both WGB and WGO could inhibit the pyroptotic markers (NLRP3, Caspase-1, GSDMD) increase and inflammatory factors release, promote the synthesis of Short-chain fatty acids (SCFAs), and the effect of WGO was more excellent than WGB. This study fully verified that the reverse effect of the dietary whole grain on BaP-induced genotoxicity, oxidative and pyroptotic injury in liver of mice, and provided a powerful theoretical support for new toxicity control dietary methods in the future.http://www.sciencedirect.com/science/article/pii/S1756464622001529Whole Grain dietBenzo[a]pyreneGenotoxicityOxidative damagePyroptosisLiver injury |
| spellingShingle | Hao Li Li Yuan Zhulin Wang Lin Shi Rui Dong Xinzhong Hu Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice Journal of Functional Foods Whole Grain diet Benzo[a]pyrene Genotoxicity Oxidative damage Pyroptosis Liver injury |
| title | Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice |
| title_full | Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice |
| title_fullStr | Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice |
| title_full_unstemmed | Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice |
| title_short | Effects of dietary whole grain buckwheat and oat on benzo[a]pyrene-induced genotoxicity, oxidative and pyroptotic injury in liver of mice |
| title_sort | effects of dietary whole grain buckwheat and oat on benzo a pyrene induced genotoxicity oxidative and pyroptotic injury in liver of mice |
| topic | Whole Grain diet Benzo[a]pyrene Genotoxicity Oxidative damage Pyroptosis Liver injury |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622001529 |
| work_keys_str_mv | AT haoli effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice AT liyuan effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice AT zhulinwang effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice AT linshi effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice AT ruidong effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice AT xinzhonghu effectsofdietarywholegrainbuckwheatandoatonbenzoapyreneinducedgenotoxicityoxidativeandpyroptoticinjuryinliverofmice |