Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies

Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies

Summary: Generating knockout mice for target molecules in specific T cell populations, without subset-specific promoters, is time-consuming and costly. Here, we describe steps for enriching mucosal-associated invariant T cells from the thymus, expanding them in vitro and performing a CRISPR-Cas9 kno...

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Bibliographic Details
Main Authors: Anastasia du Halgouet, Aurélie Darbois, Aurélia Alphonse, Thomas Yvorra, Ludovic Colombeau, Raphaël Rodriguez, Olivier Lantz, Marion Salou
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell culture
Immunology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003866

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http://www.sciencedirect.com/science/article/pii/S2666166723003866

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