SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways
Signaling lymphocytic activation molecule family 8 (SLAMF8) is involved in the negative modulation of NADPH oxidase activation. However, the impact of SLAMF8 downregulation on macrophage functionality and the microbicide mechanism remains elusive. To study this in depth, we first analyzed NADPH oxid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.910112/full |
| _version_ | 1811237041326784512 |
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| author | Salvador Romero-Pinedo Domingo I. Rojas Barros María José Ruiz-Magaña Elena Maganto-García Laura Moreno de Lara Francisco Abadía-Molina Francisco Abadía-Molina Cox Terhorst Ana C. Abadía-Molina Ana C. Abadía-Molina |
| author_facet | Salvador Romero-Pinedo Domingo I. Rojas Barros María José Ruiz-Magaña Elena Maganto-García Laura Moreno de Lara Francisco Abadía-Molina Francisco Abadía-Molina Cox Terhorst Ana C. Abadía-Molina Ana C. Abadía-Molina |
| author_sort | Salvador Romero-Pinedo |
| collection | DOAJ |
| description | Signaling lymphocytic activation molecule family 8 (SLAMF8) is involved in the negative modulation of NADPH oxidase activation. However, the impact of SLAMF8 downregulation on macrophage functionality and the microbicide mechanism remains elusive. To study this in depth, we first analyzed NADPH oxidase activation pathways in wild-type and SLAMF8-deficient macrophages upon different stimulus. Herein, we describe increased phosphorylation of the Erk1/2 and p38 MAP kinases, as well as increased phosphorylation of NADPH oxidase subunits in SLAMF8-deficient macrophages. Furthermore, using specific inhibitors, we observed that specific PI3K inhibition decreased the differences observed between wild-type and SLAMF8-deficient macrophages, stimulated with either PMA, LPS, or Salmonella typhimurium infection. Consequently, SLAMF8-deficient macrophages also showed increased recruitment of small GTPases such as Rab5 and Rab7, and the p47phox subunit to cytoplasmic Salmonella, suggesting an impairment of Salmonella-containing vacuole (SCV) progression in SLAMF8-deficient macrophages. Enhanced iNOS activation, NO production, and IL-6 expression were also observed in the absence of SLAMF8 upon Salmonella infection, either in vivo or in vitro, while overexpression of SLAMF8 in RAW264.7 macrophages showed the opposite phenotype. In addition, SLAMF8-deficient macrophages showed increased activation of Src kinases and reduced SHP-1 phosphate levels upon IFNγ and Salmonella stimuli in comparison to wild-type macrophages. In agreement with in vitro results, Salmonella clearance was augmented in SLAMF8-deficient mice compared to that in wild-type mice. Therefore, in conclusion, SLAMF8 intervention upon bacterial infection downregulates mouse macrophage activation, and confirmed that SLAMF8 receptor could be a potential therapeutic target for the treatment of severe or unresolved inflammatory conditions. |
| first_indexed | 2024-04-12T12:18:50Z |
| format | Article |
| id | doaj.art-62da8da4c04149f1bae0dea326cee612 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T12:18:50Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-62da8da4c04149f1bae0dea326cee6122022-12-22T03:33:21ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-06-011310.3389/fimmu.2022.910112910112SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K PathwaysSalvador Romero-Pinedo0Domingo I. Rojas Barros1María José Ruiz-Magaña2Elena Maganto-García3Laura Moreno de Lara4Francisco Abadía-Molina5Francisco Abadía-Molina6Cox Terhorst7Ana C. Abadía-Molina8Ana C. Abadía-Molina9Unidad de Inmunología, Instituto de Biopatología y Medicina Regenerativa (IBIMER), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainUnidad de Inmunología, Instituto de Biopatología y Medicina Regenerativa (IBIMER), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainUnidad de Inmunología, Instituto de Biopatología y Medicina Regenerativa (IBIMER), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainCentro de Biología Molecular “Severo Ochoa” Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, Madrid, SpainUnidad de Inmunología, Instituto de Biopatología y Medicina Regenerativa (IBIMER), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainDepartamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, Granada, SpainInstituto de Nutrición Y Tecnología de los Alimentos “José Mataix”, (INYTIA), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainDivision of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United StatesUnidad de Inmunología, Instituto de Biopatología y Medicina Regenerativa (IBIMER), Centro de Investigación Biomédica (CIBM), Universidad de Granada, Granada, SpainDepartamento de Bioqu´ımica y Biolog´ıa Molecular III e Inmunolog´ıa, Facultad de Medicina, Universidad de Granada, Granada, SpainSignaling lymphocytic activation molecule family 8 (SLAMF8) is involved in the negative modulation of NADPH oxidase activation. However, the impact of SLAMF8 downregulation on macrophage functionality and the microbicide mechanism remains elusive. To study this in depth, we first analyzed NADPH oxidase activation pathways in wild-type and SLAMF8-deficient macrophages upon different stimulus. Herein, we describe increased phosphorylation of the Erk1/2 and p38 MAP kinases, as well as increased phosphorylation of NADPH oxidase subunits in SLAMF8-deficient macrophages. Furthermore, using specific inhibitors, we observed that specific PI3K inhibition decreased the differences observed between wild-type and SLAMF8-deficient macrophages, stimulated with either PMA, LPS, or Salmonella typhimurium infection. Consequently, SLAMF8-deficient macrophages also showed increased recruitment of small GTPases such as Rab5 and Rab7, and the p47phox subunit to cytoplasmic Salmonella, suggesting an impairment of Salmonella-containing vacuole (SCV) progression in SLAMF8-deficient macrophages. Enhanced iNOS activation, NO production, and IL-6 expression were also observed in the absence of SLAMF8 upon Salmonella infection, either in vivo or in vitro, while overexpression of SLAMF8 in RAW264.7 macrophages showed the opposite phenotype. In addition, SLAMF8-deficient macrophages showed increased activation of Src kinases and reduced SHP-1 phosphate levels upon IFNγ and Salmonella stimuli in comparison to wild-type macrophages. In agreement with in vitro results, Salmonella clearance was augmented in SLAMF8-deficient mice compared to that in wild-type mice. Therefore, in conclusion, SLAMF8 intervention upon bacterial infection downregulates mouse macrophage activation, and confirmed that SLAMF8 receptor could be a potential therapeutic target for the treatment of severe or unresolved inflammatory conditions.https://www.frontiersin.org/articles/10.3389/fimmu.2022.910112/fullSLAMF8macrophagesSLAMFPI3K signaling pathwaySalmonella typhimurium |
| spellingShingle | Salvador Romero-Pinedo Domingo I. Rojas Barros María José Ruiz-Magaña Elena Maganto-García Laura Moreno de Lara Francisco Abadía-Molina Francisco Abadía-Molina Cox Terhorst Ana C. Abadía-Molina Ana C. Abadía-Molina SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways Frontiers in Immunology SLAMF8 macrophages SLAMF PI3K signaling pathway Salmonella typhimurium |
| title | SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways |
| title_full | SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways |
| title_fullStr | SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways |
| title_full_unstemmed | SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways |
| title_short | SLAMF8 Downregulates Mouse Macrophage Microbicidal Mechanisms via PI3K Pathways |
| title_sort | slamf8 downregulates mouse macrophage microbicidal mechanisms via pi3k pathways |
| topic | SLAMF8 macrophages SLAMF PI3K signaling pathway Salmonella typhimurium |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.910112/full |
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