Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever
Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01552/full |
| _version_ | 1828387229608706048 |
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| author | M. Gill Hartley Esther Ralph Isobel H. Norville Joann L. Prior Joann L. Prior Timothy P. Atkins Timothy P. Atkins |
| author_facet | M. Gill Hartley Esther Ralph Isobel H. Norville Joann L. Prior Joann L. Prior Timothy P. Atkins Timothy P. Atkins |
| author_sort | M. Gill Hartley |
| collection | DOAJ |
| description | Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viable bacterial load within tissues. Following recent advances in the culture methods, it has become possible to do the same with C. burnetii. Here we compare and contrast the different information gained by using PCR or viable counts to study this disease. Viable bacteria were cleared from organs much faster than previously reported when assessed by bacterial DNA, but weight loss and clinical signs improved while animals were still heavily infected. |
| first_indexed | 2024-12-10T05:52:12Z |
| format | Article |
| id | doaj.art-63057ebfe1f44e60b7abf7ac7c0f1df0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T05:52:12Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-63057ebfe1f44e60b7abf7ac7c0f1df02022-12-22T02:00:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-07-011010.3389/fmicb.2019.01552455449Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q FeverM. Gill Hartley0Esther Ralph1Isobel H. Norville2Joann L. Prior3Joann L. Prior4Timothy P. Atkins5Timothy P. Atkins6CBR, Defence Science and Technology Laboratory, Salisbury, United KingdomCBR, Defence Science and Technology Laboratory, Salisbury, United KingdomCBR, Defence Science and Technology Laboratory, Salisbury, United KingdomCBR, Defence Science and Technology Laboratory, Salisbury, United KingdomCollege of Life and Environmental Sciences, University of Exeter, Exeter, United KingdomCBR, Defence Science and Technology Laboratory, Salisbury, United KingdomCollege of Life and Environmental Sciences, University of Exeter, Exeter, United KingdomHistorically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viable bacterial load within tissues. Following recent advances in the culture methods, it has become possible to do the same with C. burnetii. Here we compare and contrast the different information gained by using PCR or viable counts to study this disease. Viable bacteria were cleared from organs much faster than previously reported when assessed by bacterial DNA, but weight loss and clinical signs improved while animals were still heavily infected.https://www.frontiersin.org/article/10.3389/fmicb.2019.01552/fullCoxiellaviabilitymouse modelPCRcounts |
| spellingShingle | M. Gill Hartley Esther Ralph Isobel H. Norville Joann L. Prior Joann L. Prior Timothy P. Atkins Timothy P. Atkins Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever Frontiers in Microbiology Coxiella viability mouse model PCR counts |
| title | Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever |
| title_full | Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever |
| title_fullStr | Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever |
| title_full_unstemmed | Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever |
| title_short | Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever |
| title_sort | comparison of pcr and viable count as a method for enumeration of bacteria in an a j mouse aerosol model of q fever |
| topic | Coxiella viability mouse model PCR counts |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01552/full |
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