Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization
A novel Fischer carbene complex has been synthesized which has tuneable reactivity owing to its dual functionality to facilitate binding with peptides and proteins via thiol moiety in presence of the amine groups of abundant lysine residues. The Fischer carbene has been so designed that it can be ma...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715622001382 |
| _version_ | 1811178127297085440 |
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| author | Namrata Ray |
| author_facet | Namrata Ray |
| author_sort | Namrata Ray |
| collection | DOAJ |
| description | A novel Fischer carbene complex has been synthesized which has tuneable reactivity owing to its dual functionality to facilitate binding with peptides and proteins via thiol moiety in presence of the amine groups of abundant lysine residues. The Fischer carbene has been so designed that it can be made the reactive terminus of a self-assembled monolayer (SAM) via Click chemistry or cross-metathesis, with retention of its activity towards thiols. Hence, using such a Fischer carbene terminated SAM, proteins may be site-specifically immobilized on surfaces, under mild condition. |
| first_indexed | 2024-04-11T06:12:55Z |
| format | Article |
| id | doaj.art-6359c05226d946909a206413c13f210d |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-04-11T06:12:55Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-6359c05226d946909a206413c13f210d2022-12-22T04:41:09ZengElsevierResults in Chemistry2211-71562022-01-014100419Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilizationNamrata Ray0Address: Department of Chemistry, Santipur College, N.S. Road Santipur, Nadia 741404, India.; Department of Organic Chemistry, Indian Association for the Cultivation of Science, 2A & 2B Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, IndiaA novel Fischer carbene complex has been synthesized which has tuneable reactivity owing to its dual functionality to facilitate binding with peptides and proteins via thiol moiety in presence of the amine groups of abundant lysine residues. The Fischer carbene has been so designed that it can be made the reactive terminus of a self-assembled monolayer (SAM) via Click chemistry or cross-metathesis, with retention of its activity towards thiols. Hence, using such a Fischer carbene terminated SAM, proteins may be site-specifically immobilized on surfaces, under mild condition.http://www.sciencedirect.com/science/article/pii/S2211715622001382Fischer carbeneThiolProteinImmobilization |
| spellingShingle | Namrata Ray Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization Results in Chemistry Fischer carbene Thiol Protein Immobilization |
| title | Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization |
| title_full | Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization |
| title_fullStr | Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization |
| title_full_unstemmed | Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization |
| title_short | Design of a novel Fischer carbene complex which can facilitate thiol mediated site-specific protein immobilization |
| title_sort | design of a novel fischer carbene complex which can facilitate thiol mediated site specific protein immobilization |
| topic | Fischer carbene Thiol Protein Immobilization |
| url | http://www.sciencedirect.com/science/article/pii/S2211715622001382 |
| work_keys_str_mv | AT namrataray designofanovelfischercarbenecomplexwhichcanfacilitatethiolmediatedsitespecificproteinimmobilization |