Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells
The main products formed from glucosinolates from Brassicales plants are isothiocyanates (ITCs) and nitriles. ITCs are known for their diverse bioactive potential, knowledge about the bioactivity of food-borne nitriles is poor, though. Anti-inflammation by benzyl ITC (BITC) and its nitrile has not s...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616000906 |
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| author | Corinna Herz Melinda-Rita Márton Hoai Thi Thu Tran Carsten Gründemann Jana Schell Evelyn Lamy |
| author_facet | Corinna Herz Melinda-Rita Márton Hoai Thi Thu Tran Carsten Gründemann Jana Schell Evelyn Lamy |
| author_sort | Corinna Herz |
| collection | DOAJ |
| description | The main products formed from glucosinolates from Brassicales plants are isothiocyanates (ITCs) and nitriles. ITCs are known for their diverse bioactive potential, knowledge about the bioactivity of food-borne nitriles is poor, though. Anti-inflammation by benzyl ITC (BITC) and its nitrile has not so far been studied in human immune cells. Here, the potential of BITC to inhibit the lipopolysaccharide (LPS) induced inflammatory response in human peripheral mononuclear blood cells (PBMC) was analysed in comparison to benzyl nitrile (BCN).Our data show that BITC strongly interferes with the cellular pro-inflammatory response in terms of dual COX and LOX pathway inhibition and reduced the activation and function of activated T lymphocytes. In contrast, BCN did not influence prostaglandin PGE2 release from PBMC. The inactivity of the glucosinolate product BCN must be considered when Brassicales plants are intended for use against inflammatory diseases. |
| first_indexed | 2024-12-24T11:20:48Z |
| format | Article |
| id | doaj.art-6375c306a84b42e89346312a91a190b1 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T11:20:48Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6375c306a84b42e89346312a91a190b12022-12-21T16:58:15ZengElsevierJournal of Functional Foods1756-46462016-05-0123135143Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cellsCorinna Herz0Melinda-Rita Márton1Hoai Thi Thu Tran2Carsten Gründemann3Jana Schell4Evelyn Lamy5Molecular Preventive Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, GermanyMolecular Preventive Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, GermanyMolecular Preventive Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, GermanyCenter for Complementary Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, GermanyMolecular Preventive Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, GermanyMolecular Preventive Medicine, Institute of Environmental Health Sciences, University of Freiburg – Medical Center, Breisacher Strasse 115b, 79106 Freiburg, Germany; Corresponding author. Molecular Preventive Medicine, Institute for Prevention and Cancer Epidemiology, University of Freiburg – Medical Center, Elsässerstr. 2, 79110 Freiburg, Germany. Tel.: +49(0)761 270 82150; fax: +49(0)761 270 82130.The main products formed from glucosinolates from Brassicales plants are isothiocyanates (ITCs) and nitriles. ITCs are known for their diverse bioactive potential, knowledge about the bioactivity of food-borne nitriles is poor, though. Anti-inflammation by benzyl ITC (BITC) and its nitrile has not so far been studied in human immune cells. Here, the potential of BITC to inhibit the lipopolysaccharide (LPS) induced inflammatory response in human peripheral mononuclear blood cells (PBMC) was analysed in comparison to benzyl nitrile (BCN).Our data show that BITC strongly interferes with the cellular pro-inflammatory response in terms of dual COX and LOX pathway inhibition and reduced the activation and function of activated T lymphocytes. In contrast, BCN did not influence prostaglandin PGE2 release from PBMC. The inactivity of the glucosinolate product BCN must be considered when Brassicales plants are intended for use against inflammatory diseases.http://www.sciencedirect.com/science/article/pii/S1756464616000906Benzyl isothiocyanateBITCBrassicalesAnti-inflammatory effect in primary human PBMCBenzyl nitrileDual LOX and COX pathway inhibitor |
| spellingShingle | Corinna Herz Melinda-Rita Márton Hoai Thi Thu Tran Carsten Gründemann Jana Schell Evelyn Lamy Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells Journal of Functional Foods Benzyl isothiocyanate BITC Brassicales Anti-inflammatory effect in primary human PBMC Benzyl nitrile Dual LOX and COX pathway inhibitor |
| title | Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells |
| title_full | Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells |
| title_fullStr | Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells |
| title_full_unstemmed | Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells |
| title_short | Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells |
| title_sort | benzyl isothiocyanate but not benzyl nitrile from brassicales plants dually blocks the cox and lox pathway in primary human immune cells |
| topic | Benzyl isothiocyanate BITC Brassicales Anti-inflammatory effect in primary human PBMC Benzyl nitrile Dual LOX and COX pathway inhibitor |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616000906 |
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