Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth
Abstract Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro‐scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202308478 |
| _version_ | 1797255004907634688 |
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| author | Junxuan Ma Janick Eglauf Sibylle Grad Mauro Alini Tiziano Serra |
| author_facet | Junxuan Ma Janick Eglauf Sibylle Grad Mauro Alini Tiziano Serra |
| author_sort | Junxuan Ma |
| collection | DOAJ |
| description | Abstract Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro‐scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within a collagen matrix. This results in a densely packed multicellular system mimicking the native DRG tissue morphology and a controlled AF‐neuron distance. Such a multicellular organisation is essential to evolve populational‐level cellular functions and in vivo‐like morphologies. Pro‐inflammatory cytokine‐primed AF demonstrates its neurotrophic and neurotropic effects on nociceptor axons. Both effects are dependent on the AF‐neuron distance underpinning the role of recapitulating inter‐tissue/organ anatomical proximity when investigating their crosstalk. This is the first in vitro model studying AF nerve ingrowth by engineering mature and large animal tissues in a morphologically and physiologically relevant environment. The new approach can be used to biofabricate multi‐tissue/organ models for untangling pathophysiological conditions and develop novel therapies. |
| first_indexed | 2024-04-24T21:58:58Z |
| format | Article |
| id | doaj.art-63db4b9bb14e43dd8bc623c545f70386 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-24T21:58:58Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-63db4b9bb14e43dd8bc623c545f703862024-03-20T12:56:12ZengWileyAdvanced Science2198-38442024-03-011111n/an/a10.1002/advs.202308478Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve IngrowthJunxuan Ma0Janick Eglauf1Sibylle Grad2Mauro Alini3Tiziano Serra4AO Research Institute Clavadelerstrasse 8 Davos 7270 SwitzerlandAO Research Institute Clavadelerstrasse 8 Davos 7270 SwitzerlandAO Research Institute Clavadelerstrasse 8 Davos 7270 SwitzerlandAO Research Institute Clavadelerstrasse 8 Davos 7270 SwitzerlandAO Research Institute Clavadelerstrasse 8 Davos 7270 SwitzerlandAbstract Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro‐scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within a collagen matrix. This results in a densely packed multicellular system mimicking the native DRG tissue morphology and a controlled AF‐neuron distance. Such a multicellular organisation is essential to evolve populational‐level cellular functions and in vivo‐like morphologies. Pro‐inflammatory cytokine‐primed AF demonstrates its neurotrophic and neurotropic effects on nociceptor axons. Both effects are dependent on the AF‐neuron distance underpinning the role of recapitulating inter‐tissue/organ anatomical proximity when investigating their crosstalk. This is the first in vitro model studying AF nerve ingrowth by engineering mature and large animal tissues in a morphologically and physiologically relevant environment. The new approach can be used to biofabricate multi‐tissue/organ models for untangling pathophysiological conditions and develop novel therapies.https://doi.org/10.1002/advs.202308478acoustic assemblyadvanced in vitro modelsalternatives to animal testingmulticellular systemsensory nerve ingrowth |
| spellingShingle | Junxuan Ma Janick Eglauf Sibylle Grad Mauro Alini Tiziano Serra Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth Advanced Science acoustic assembly advanced in vitro models alternatives to animal testing multicellular system sensory nerve ingrowth |
| title | Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth |
| title_full | Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth |
| title_fullStr | Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth |
| title_full_unstemmed | Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth |
| title_short | Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth |
| title_sort | engineering sensory ganglion multicellular system to model tissue nerve ingrowth |
| topic | acoustic assembly advanced in vitro models alternatives to animal testing multicellular system sensory nerve ingrowth |
| url | https://doi.org/10.1002/advs.202308478 |
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