Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua.
IntroductionPolygonatum cyrtonema Hua. (PC) is a traditional Chinese herb with a history of use in both food and medicine. For clinical use, processed PC pieces are most commonly used, while present research has focused on crude PC polysaccharides (PCPs).MethodsIn this study, a new polysaccharide, P...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1272977/full |
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| author | Yuanyuan Zhao Zhen Wang Ranze Fu Ruonan Xie Bin Wang Bin Wang Qinglin Li |
| author_facet | Yuanyuan Zhao Zhen Wang Ranze Fu Ruonan Xie Bin Wang Bin Wang Qinglin Li |
| author_sort | Yuanyuan Zhao |
| collection | DOAJ |
| description | IntroductionPolygonatum cyrtonema Hua. (PC) is a traditional Chinese herb with a history of use in both food and medicine. For clinical use, processed PC pieces are most commonly used, while present research has focused on crude PC polysaccharides (PCPs).MethodsIn this study, a new polysaccharide, PCP-F1, with a molecular weight of 37.46 kDa, was separated from four-time processed PCPs by column chromatography and evaluated by antioxidant activity. It was composed of glucose, mannose, galactose, rhamnose, and galacturonic acid with a molar ratio of 3.5: 2.5: 1.3: 1.8: 0.8.Results and DiscussionThe methylation analysis and two-dimensional NMR measurement revealed that the configuration of PCP-F1 contained nine residues in the primary structural unit by the chain of →3)-α-D-Glcp, →2)-α-D-Glcp (6→, →1)-ꞵ-D-Glcp (2→, →2)-α-D-GalAp (3,4→, →1) -ꞵ-D-Manp (3→, →2)-α-D-Glcp (3→, branched for →3)-α-D-Glcp, →2)-ꞵ-D-Galp (4→, →1)-ꞵ-D-Glcp (2→, →2,4)-α-D-Manp (6→, →3)-α-L-Rhap (4→. Radical scavenging assays indicated that PCP-F1 could scavenge radicals with a high scavenging rate, suggesting PCP-F1 possesses good antioxidant activity. The study confirms the importance of processed PC and offers the potential for exploiting it as a functional food. |
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| language | English |
| last_indexed | 2024-03-12T02:28:56Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-63db62fc3b0843bb811e7a6a89a569ca2023-09-05T10:48:12ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-09-011010.3389/fnut.2023.12729771272977Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua.Yuanyuan Zhao0Zhen Wang1Ranze Fu2Ruonan Xie3Bin Wang4Bin Wang5Qinglin Li6Key Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaInstitute of Pharmaceutical Chemistry, Anhui Academy of Chinese Medicine, Hefei, Anhui, ChinaKey Laboratory of Xin'an Medicine of the Ministry of Education, Anhui University of Chinese Medicine, Hefei, ChinaIntroductionPolygonatum cyrtonema Hua. (PC) is a traditional Chinese herb with a history of use in both food and medicine. For clinical use, processed PC pieces are most commonly used, while present research has focused on crude PC polysaccharides (PCPs).MethodsIn this study, a new polysaccharide, PCP-F1, with a molecular weight of 37.46 kDa, was separated from four-time processed PCPs by column chromatography and evaluated by antioxidant activity. It was composed of glucose, mannose, galactose, rhamnose, and galacturonic acid with a molar ratio of 3.5: 2.5: 1.3: 1.8: 0.8.Results and DiscussionThe methylation analysis and two-dimensional NMR measurement revealed that the configuration of PCP-F1 contained nine residues in the primary structural unit by the chain of →3)-α-D-Glcp, →2)-α-D-Glcp (6→, →1)-ꞵ-D-Glcp (2→, →2)-α-D-GalAp (3,4→, →1) -ꞵ-D-Manp (3→, →2)-α-D-Glcp (3→, branched for →3)-α-D-Glcp, →2)-ꞵ-D-Galp (4→, →1)-ꞵ-D-Glcp (2→, →2,4)-α-D-Manp (6→, →3)-α-L-Rhap (4→. Radical scavenging assays indicated that PCP-F1 could scavenge radicals with a high scavenging rate, suggesting PCP-F1 possesses good antioxidant activity. The study confirms the importance of processed PC and offers the potential for exploiting it as a functional food.https://www.frontiersin.org/articles/10.3389/fnut.2023.1272977/fullpolysaccharideP. cyrtonema Hua.steaming and shiningstructural characterizationantioxidant activityfunctional food |
| spellingShingle | Yuanyuan Zhao Zhen Wang Ranze Fu Ruonan Xie Bin Wang Bin Wang Qinglin Li Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. Frontiers in Nutrition polysaccharide P. cyrtonema Hua. steaming and shining structural characterization antioxidant activity functional food |
| title | Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. |
| title_full | Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. |
| title_fullStr | Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. |
| title_full_unstemmed | Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. |
| title_short | Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua. |
| title_sort | structural characterization and antioxidant activity of processed polysaccharides pcp f1 from polygonatum cyrtonema hua |
| topic | polysaccharide P. cyrtonema Hua. steaming and shining structural characterization antioxidant activity functional food |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1272977/full |
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