Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure

Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure

Prime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the pegRNA secondary structure was altered to increa...

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Bibliographic Details
Main Authors: Xiaosa Li, Lina Zhou, Bao-Qing Gao, Guangye Li, Xiao Wang, Ying Wang, Jia Wei, Wenyan Han, Zixian Wang, Jifang Li, Runze Gao, Junjie Zhu, Wenchao Xu, Jing Wu, Bei Yang, Xiaodong Sun, Li Yang, Jia Chen
Format: Article
Language:English
Published: Nature Portfolio 2022-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-29339-9

Internet

https://doi.org/10.1038/s41467-022-29339-9

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