Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure
Prime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the pegRNA secondary structure was altered to increa...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-29339-9 |
| _version_ | 1811273063415676928 |
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| author | Xiaosa Li Lina Zhou Bao-Qing Gao Guangye Li Xiao Wang Ying Wang Jia Wei Wenyan Han Zixian Wang Jifang Li Runze Gao Junjie Zhu Wenchao Xu Jing Wu Bei Yang Xiaodong Sun Li Yang Jia Chen |
| author_facet | Xiaosa Li Lina Zhou Bao-Qing Gao Guangye Li Xiao Wang Ying Wang Jia Wei Wenyan Han Zixian Wang Jifang Li Runze Gao Junjie Zhu Wenchao Xu Jing Wu Bei Yang Xiaodong Sun Li Yang Jia Chen |
| author_sort | Xiaosa Li |
| collection | DOAJ |
| description | Prime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the pegRNA secondary structure was altered to increase indel-editing efficiency. |
| first_indexed | 2024-04-12T22:51:13Z |
| format | Article |
| id | doaj.art-63feadad23c44c5db5815b59a02b9ad1 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T22:51:13Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-63feadad23c44c5db5815b59a02b9ad12022-12-22T03:13:20ZengNature PortfolioNature Communications2041-17232022-03-011311910.1038/s41467-022-29339-9Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structureXiaosa Li0Lina Zhou1Bao-Qing Gao2Guangye Li3Xiao Wang4Ying Wang5Jia Wei6Wenyan Han7Zixian Wang8Jifang Li9Runze Gao10Junjie Zhu11Wenchao Xu12Jing Wu13Bei Yang14Xiaodong Sun15Li Yang16Jia Chen17Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of SciencesGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of SciencesCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of SciencesGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityShanghai Clinical Research and Trial CenterDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineCenter for Molecular Medicine, Children’s Hospital, Fudan University and Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan UniversityGene Editing Center, School of Life Science and Technology, ShanghaiTech UniversityPrime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the pegRNA secondary structure was altered to increase indel-editing efficiency.https://doi.org/10.1038/s41467-022-29339-9 |
| spellingShingle | Xiaosa Li Lina Zhou Bao-Qing Gao Guangye Li Xiao Wang Ying Wang Jia Wei Wenyan Han Zixian Wang Jifang Li Runze Gao Junjie Zhu Wenchao Xu Jing Wu Bei Yang Xiaodong Sun Li Yang Jia Chen Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure Nature Communications |
| title | Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure |
| title_full | Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure |
| title_fullStr | Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure |
| title_full_unstemmed | Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure |
| title_short | Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure |
| title_sort | highly efficient prime editing by introducing same sense mutations in pegrna or stabilizing its structure |
| url | https://doi.org/10.1038/s41467-022-29339-9 |
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