Combining Recombinase-Mediated Cassette Exchange Strategy with Quantitative Proteomic and Phosphoproteomic Analyses to Inspect Intracellular Functions of the Tumor Suppressor Galectin-4 in Colorectal Cancer Cells

Combining Recombinase-Mediated Cassette Exchange Strategy with Quantitative Proteomic and Phosphoproteomic Analyses to Inspect Intracellular Functions of the Tumor Suppressor Galectin-4 in Colorectal Cancer Cells

Galectin-4 (Gal4) has been suggested to function as a tumor suppressor in colorectal cancer (CRC). In order to systematically explore its function in CRC, we established a CRC cell line where Gal4 expression can be regulated via the doxycycline (dox)-inducible expression of a single copy wildtype &l...

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Bibliographic Details
Main Authors: Malwina Michalak, Viola Golde, Dominik Helm, Herbert Kaltner, Johannes Gebert, Jürgen Kopitz
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:International Journal of Molecular Sciences
Subjects:
Galectin 4
proteomics
phosphoproteomics
colorectal cancer
Tet-On system
Online Access:https://www.mdpi.com/1422-0067/23/12/6414

Internet

https://www.mdpi.com/1422-0067/23/12/6414

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