Spectral characteristics of the mutant form GGBP/H152C of D-glucose/D-galactose-binding protein labeled with fluorescent dye BADAN: influence of external factors

Spectral characteristics of the mutant form GGBP/H152C of D-glucose/D-galactose-binding protein labeled with fluorescent dye BADAN: influence of external factors

The mutant form GGBP/H152C of the D-glucose/D-galactose-binding protein with the solvatochromic dye BADAN linked to cysteine residue Cys 152 can be used as a potential base for a sensitive element of glucose biosensor system. We investigated the influence of various external factors on the physical-...

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Bibliographic Details
Main Authors: Alexander V. Fonin, Olga V. Stepanenko, Olga I. Povarova, Catherine A. Volova, Elizaveta M. Philippova, Grigory S. Bublikov, Irina M. Kuznetsova, Alexander P. Demchenko, Konstantin K. Turoverov
Format: Article
Language:English
Published: PeerJ Inc. 2014-03-01
Series:PeerJ
Subjects:
D-glucose/D-galactose-binding protein
Fluorescent dye BADAN
Protein stability
Biosensors
Glucose binding constant
Online Access:https://peerj.com/articles/275.pdf

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https://peerj.com/articles/275.pdf

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