Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries
Livestock data on antimicrobial resistance (AMR) are commonly collected from bacterial populations of clinical and non-clinical isolates. In contrast to data on non-clinical isolates from livestock, data on clinical isolates are not harmonized in Europe. The Normalized Resistance Interpretation (NRI...
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/4/678 |
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| author | Octavio Mesa-Varona Rodolphe Mader Martina Velasova Jean-Yves Madec Sophie A. Granier Agnes Perrin-Guyomard Madelaine Norstrom Heike Kaspar Mirjam Grobbel Eric Jouy Muna F. Anjum Bernd-Alois Tenhagen |
| author_facet | Octavio Mesa-Varona Rodolphe Mader Martina Velasova Jean-Yves Madec Sophie A. Granier Agnes Perrin-Guyomard Madelaine Norstrom Heike Kaspar Mirjam Grobbel Eric Jouy Muna F. Anjum Bernd-Alois Tenhagen |
| author_sort | Octavio Mesa-Varona |
| collection | DOAJ |
| description | Livestock data on antimicrobial resistance (AMR) are commonly collected from bacterial populations of clinical and non-clinical isolates. In contrast to data on non-clinical isolates from livestock, data on clinical isolates are not harmonized in Europe. The Normalized Resistance Interpretation (NRI) method was applied to overcome the lack of harmonization of laboratory methods and interpretation rules between monitoring systems. Statistical analyses were performed to identify associations between the isolate type (clinical vs. non-clinical) and resistance to four antimicrobials (ampicillin, tetracycline, gentamicin, and nalidixic acid) per animal category in Germany and France. Additional statistical analyses comparing clinical and non-clinical isolates were performed with the available data on the same antimicrobial panel and animal categories from the UK and Norway. Higher resistance prevalence was found in clinical isolates compared to non-clinical isolates from calves to all antimicrobials included in Germany and France. It was also found for gentamicin in broilers from France. In contrast, in broilers and turkeys from Germany and France and in broilers from the UK, a higher resistance level to ampicillin and tetracycline in non-clinical isolates was encountered. This was also found in resistance to gentamicin in isolates from turkeys in Germany. Resistance differed within countries and across years, which was partially in line with differences in antimicrobial use patterns. Differences in AMR between clinical and non-clinical isolates of <i>Escherichia coli</i> are associated with animal category (broiler, calf, and turkey) and specific antimicrobials. The NRI method allowed comparing results of non-harmonized AMR systems and might be useful until international harmonization is achieved. |
| first_indexed | 2024-03-10T12:55:06Z |
| format | Article |
| id | doaj.art-641e9aceca3544d6adcdd01ee0208a4c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:55:06Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-641e9aceca3544d6adcdd01ee0208a4c2023-11-21T11:58:30ZengMDPI AGMicroorganisms2076-26072021-03-019467810.3390/microorganisms9040678Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European CountriesOctavio Mesa-Varona0Rodolphe Mader1Martina Velasova2Jean-Yves Madec3Sophie A. Granier4Agnes Perrin-Guyomard5Madelaine Norstrom6Heike Kaspar7Mirjam Grobbel8Eric Jouy9Muna F. Anjum10Bernd-Alois Tenhagen11German Federal Institute for Risk Assessment (BfR), Department Biological Safety, 10589 Berlin, GermanyFrench Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Lyon, Antibiotic Resistance and Bacterial Virulence Unit, University of Lyon, 31 avenue Tony Garnier, 69007 Lyon, FranceAnimal and Plant Health Agency (APHA), Department of Epidemiological Sciences, Addlestone, Surrey KT15 3NB, UKFrench Agency for Food, Environmental and Occupational Health and Safety (ANSES), Laboratory of Lyon, Antibiotic Resistance and Bacterial Virulence Unit, University of Lyon, 31 avenue Tony Garnier, 69007 Lyon, FranceFrench Agency for Food, Environmental and Occupational Health and Safety (ANSES), Fougeres Laboratory, 35133 Fougeres, FranceFrench Agency for Food, Environmental and Occupational Health and Safety (ANSES), Fougeres Laboratory, 35133 Fougeres, FranceNorwegian Veterinary Institute (NVI), Department of Animal Health and Food Safety, Research Section Food Safety and Animal Health, 0454 Oslo, NorwayFederal Office of Consumer Protection and Food Safety (BVL), Reference Laboratories, Resistance to Antibiotics Unit Monitoring of Resistance to Antibiotics, Department Method Standardisation, 12277 Berlin, GermanyGerman Federal Institute for Risk Assessment (BfR), Department Biological Safety, 10589 Berlin, GermanyFrench Agency for Food, Environmental and Occupational Health and Safety (ANSES), Ploufragan-Plouzané-Niort Laboratory, 22440 Ploufragan, FranceAnimal and Plant Health Agency (APHA), Department of Bacteriology, Addlestone, Surrey KT15 3NB, UKGerman Federal Institute for Risk Assessment (BfR), Department Biological Safety, 10589 Berlin, GermanyLivestock data on antimicrobial resistance (AMR) are commonly collected from bacterial populations of clinical and non-clinical isolates. In contrast to data on non-clinical isolates from livestock, data on clinical isolates are not harmonized in Europe. The Normalized Resistance Interpretation (NRI) method was applied to overcome the lack of harmonization of laboratory methods and interpretation rules between monitoring systems. Statistical analyses were performed to identify associations between the isolate type (clinical vs. non-clinical) and resistance to four antimicrobials (ampicillin, tetracycline, gentamicin, and nalidixic acid) per animal category in Germany and France. Additional statistical analyses comparing clinical and non-clinical isolates were performed with the available data on the same antimicrobial panel and animal categories from the UK and Norway. Higher resistance prevalence was found in clinical isolates compared to non-clinical isolates from calves to all antimicrobials included in Germany and France. It was also found for gentamicin in broilers from France. In contrast, in broilers and turkeys from Germany and France and in broilers from the UK, a higher resistance level to ampicillin and tetracycline in non-clinical isolates was encountered. This was also found in resistance to gentamicin in isolates from turkeys in Germany. Resistance differed within countries and across years, which was partially in line with differences in antimicrobial use patterns. Differences in AMR between clinical and non-clinical isolates of <i>Escherichia coli</i> are associated with animal category (broiler, calf, and turkey) and specific antimicrobials. The NRI method allowed comparing results of non-harmonized AMR systems and might be useful until international harmonization is achieved.https://www.mdpi.com/2076-2607/9/4/678AMRclinical isolatesnon-clinical isolatesbroilerturkeycalf |
| spellingShingle | Octavio Mesa-Varona Rodolphe Mader Martina Velasova Jean-Yves Madec Sophie A. Granier Agnes Perrin-Guyomard Madelaine Norstrom Heike Kaspar Mirjam Grobbel Eric Jouy Muna F. Anjum Bernd-Alois Tenhagen Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries Microorganisms AMR clinical isolates non-clinical isolates broiler turkey calf |
| title | Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries |
| title_full | Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries |
| title_fullStr | Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries |
| title_full_unstemmed | Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries |
| title_short | Comparison of Phenotypical Antimicrobial Resistance between Clinical and Non-Clinical <i>E. coli</i> Isolates from Broilers, Turkeys and Calves in Four European Countries |
| title_sort | comparison of phenotypical antimicrobial resistance between clinical and non clinical i e coli i isolates from broilers turkeys and calves in four european countries |
| topic | AMR clinical isolates non-clinical isolates broiler turkey calf |
| url | https://www.mdpi.com/2076-2607/9/4/678 |
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