Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons
Abstract Background Our published data have indicated that the omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) provides beneficial effects by attenuating neuronal damage induced by interleukin-1β (IL-1β), and up-regulation of the expression of brain-derived neurotrophic factor (BDNF)...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Neuroscience |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12868-018-0455-7 |
| _version_ | 1818610478482456576 |
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| author | YiLong Dong KangJing Pu WenJing Duan HuiCheng Chen LiXing Chen YanMei Wang |
| author_facet | YiLong Dong KangJing Pu WenJing Duan HuiCheng Chen LiXing Chen YanMei Wang |
| author_sort | YiLong Dong |
| collection | DOAJ |
| description | Abstract Background Our published data have indicated that the omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) provides beneficial effects by attenuating neuronal damage induced by interleukin-1β (IL-1β), and up-regulation of the expression of brain-derived neurotrophic factor (BDNF) represents a crucial part in the neuroprotective effect of EPA. However, the mechanisms of how EPA regulates BDNF expression remains incompletely understood. The present study investigated the role of Akt/CREB signaling in the effect of EPA on BDNF expression and its neuroprotective effect. Results The present results showed that IL-1β reduced hippocampal neuronal viability and that EPA showed a concentration-dependent neuroprotective effect, but the neuroprotective effects of EPA were abolished by inhibition of Akt using KRX-0401, an inhibitor of Akt. Treatment of hippocampal neurons with EPA also ameliorated the decrease in Akt and CREB phosphorylation induced by IL-1β and BDNF down-regulation mediated by IL-1β. However, inhibition of Akt reversed the effect of EPA on levels of p-Akt, p-CREB, and BDNF. Conclusions Our data indicate that EPA elicited neuroprotection toward IL-1β-induced cell damage and BDNF decrease and that its effects potentially occurred via the Akt/CREB signaling pathway. |
| first_indexed | 2024-12-16T15:15:04Z |
| format | Article |
| id | doaj.art-64222d0c51b445d4aa31b94e0a778f84 |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-16T15:15:04Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-64222d0c51b445d4aa31b94e0a778f842022-12-21T22:26:51ZengBMCBMC Neuroscience1471-22022018-09-011911810.1186/s12868-018-0455-7Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neuronsYiLong Dong0KangJing Pu1WenJing Duan2HuiCheng Chen3LiXing Chen4YanMei Wang5School of Medicine, Yunnan UniversitySchool of Medicine, Yunnan UniversityThe First Affiliated Hospital of Kunming Medical UniversitySchool of Medicine, Yunnan UniversityThe First Affiliated Hospital of Kunming Medical UniversityThe First Affiliated Hospital of Kunming Medical UniversityAbstract Background Our published data have indicated that the omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) provides beneficial effects by attenuating neuronal damage induced by interleukin-1β (IL-1β), and up-regulation of the expression of brain-derived neurotrophic factor (BDNF) represents a crucial part in the neuroprotective effect of EPA. However, the mechanisms of how EPA regulates BDNF expression remains incompletely understood. The present study investigated the role of Akt/CREB signaling in the effect of EPA on BDNF expression and its neuroprotective effect. Results The present results showed that IL-1β reduced hippocampal neuronal viability and that EPA showed a concentration-dependent neuroprotective effect, but the neuroprotective effects of EPA were abolished by inhibition of Akt using KRX-0401, an inhibitor of Akt. Treatment of hippocampal neurons with EPA also ameliorated the decrease in Akt and CREB phosphorylation induced by IL-1β and BDNF down-regulation mediated by IL-1β. However, inhibition of Akt reversed the effect of EPA on levels of p-Akt, p-CREB, and BDNF. Conclusions Our data indicate that EPA elicited neuroprotection toward IL-1β-induced cell damage and BDNF decrease and that its effects potentially occurred via the Akt/CREB signaling pathway.http://link.springer.com/article/10.1186/s12868-018-0455-7Eicosapentaenoic acidBrain-derived neurotrophic factorAktInterleukin-1β |
| spellingShingle | YiLong Dong KangJing Pu WenJing Duan HuiCheng Chen LiXing Chen YanMei Wang Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons BMC Neuroscience Eicosapentaenoic acid Brain-derived neurotrophic factor Akt Interleukin-1β |
| title | Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons |
| title_full | Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons |
| title_fullStr | Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons |
| title_full_unstemmed | Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons |
| title_short | Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1β-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons |
| title_sort | involvement of akt creb signaling pathways in the protective effect of epa against interleukin 1β induced cytotoxicity and bdnf down regulation in cultured rat hippocampal neurons |
| topic | Eicosapentaenoic acid Brain-derived neurotrophic factor Akt Interleukin-1β |
| url | http://link.springer.com/article/10.1186/s12868-018-0455-7 |
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