Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression
Abstract Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis. Tumour invasion and metastasis that affect prognosis are result of a set of complex events that cells with invasive p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13000-021-01090-7 |
| _version_ | 1819291185540235264 |
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| author | Geovanni Pereira Mitre Karolyny Martins Balbinot André Luis Ribeiro Ribeiro Maria Sueli da Silva Kataoka Sérgio de Melo Alves Júnior João de Jesus Viana Pinheiro |
| author_facet | Geovanni Pereira Mitre Karolyny Martins Balbinot André Luis Ribeiro Ribeiro Maria Sueli da Silva Kataoka Sérgio de Melo Alves Júnior João de Jesus Viana Pinheiro |
| author_sort | Geovanni Pereira Mitre |
| collection | DOAJ |
| description | Abstract Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis. Tumour invasion and metastasis that affect prognosis are result of a set of complex events that cells with invasive potential use to spread to other regions. These cells use several mechanisms to invade tissues, including a type of finger-like membrane protrusion called invadopodia. This study aims to investigate the immunoexpression of invaopodia related-proteins TKs5, cortactin, TKs4 and MT1-MMP in OSCC and correlate it to clinicopathological data. Methods An immunohistochemical evaluation of fifty cases of OSCCs and 20 cases of oral mucosa (OM) were assessed. The expression of invadopodia proteins were analysed in comparison to normal tissue (OM) and correlated to different clinical-stage and histological grade of OSCC. Results TKs5, cortactin, TKs4 and MT1-MMP were significantly overexpressed in OSCC when compared to OM (p < 0.0001). Among tumour stages, TKs5 showed a statistical difference in immunolabelling between stage I and III (p = 0.026). Cortactin immunolabelling was statistically higher in grade I than in grade II and III. No differences were seen on TKs4 expression based on tumour staging or grading. MT1-MMP was higher expressed and showed statistical difference between stages I and III and grades I compared to II and III. Conclusions The invadopodia related-proteins were found to be overexpressed in OSCC when compared to OM, suggesting invadopodia formation and activity. Besides overexpressed in OSCC, cortactin, TKs4 and TKs5 showed no or ambiguous differences in protein expression when compared among clinical-stages or histological grades groups. Conversely, the expression of MT1-MMP increased in advanced stages and less differentiated tumours, suggesting MT1-MMP expression as a promising prognostic marker in OSCC. |
| first_indexed | 2024-12-24T03:34:37Z |
| format | Article |
| id | doaj.art-642638c4bae040e8ad0ff0951f6d75dd |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-24T03:34:37Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-642638c4bae040e8ad0ff0951f6d75dd2022-12-21T17:17:07ZengBMCDiagnostic Pathology1746-15962021-04-0116111010.1186/s13000-021-01090-7Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progressionGeovanni Pereira Mitre0Karolyny Martins Balbinot1André Luis Ribeiro Ribeiro2Maria Sueli da Silva Kataoka3Sérgio de Melo Alves Júnior4João de Jesus Viana Pinheiro5Laboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáLaboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáLaboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáLaboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáLaboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáLaboratory of Histopathology and Immunohistochemistry, School of Dentistry, Cell Culture Laboratory, Federal University of ParáAbstract Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis. Tumour invasion and metastasis that affect prognosis are result of a set of complex events that cells with invasive potential use to spread to other regions. These cells use several mechanisms to invade tissues, including a type of finger-like membrane protrusion called invadopodia. This study aims to investigate the immunoexpression of invaopodia related-proteins TKs5, cortactin, TKs4 and MT1-MMP in OSCC and correlate it to clinicopathological data. Methods An immunohistochemical evaluation of fifty cases of OSCCs and 20 cases of oral mucosa (OM) were assessed. The expression of invadopodia proteins were analysed in comparison to normal tissue (OM) and correlated to different clinical-stage and histological grade of OSCC. Results TKs5, cortactin, TKs4 and MT1-MMP were significantly overexpressed in OSCC when compared to OM (p < 0.0001). Among tumour stages, TKs5 showed a statistical difference in immunolabelling between stage I and III (p = 0.026). Cortactin immunolabelling was statistically higher in grade I than in grade II and III. No differences were seen on TKs4 expression based on tumour staging or grading. MT1-MMP was higher expressed and showed statistical difference between stages I and III and grades I compared to II and III. Conclusions The invadopodia related-proteins were found to be overexpressed in OSCC when compared to OM, suggesting invadopodia formation and activity. Besides overexpressed in OSCC, cortactin, TKs4 and TKs5 showed no or ambiguous differences in protein expression when compared among clinical-stages or histological grades groups. Conversely, the expression of MT1-MMP increased in advanced stages and less differentiated tumours, suggesting MT1-MMP expression as a promising prognostic marker in OSCC.https://doi.org/10.1186/s13000-021-01090-7Oral squamous cell carcinomaImmunohistochemistryInvadopodiaNeoplasm InvasivenessPathology |
| spellingShingle | Geovanni Pereira Mitre Karolyny Martins Balbinot André Luis Ribeiro Ribeiro Maria Sueli da Silva Kataoka Sérgio de Melo Alves Júnior João de Jesus Viana Pinheiro Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression Diagnostic Pathology Oral squamous cell carcinoma Immunohistochemistry Invadopodia Neoplasm Invasiveness Pathology |
| title | Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression |
| title_full | Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression |
| title_fullStr | Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression |
| title_full_unstemmed | Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression |
| title_short | Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression |
| title_sort | key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of mt1 mmp in the tumoral progression |
| topic | Oral squamous cell carcinoma Immunohistochemistry Invadopodia Neoplasm Invasiveness Pathology |
| url | https://doi.org/10.1186/s13000-021-01090-7 |
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