Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

Abstract Background The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the in...

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Bibliographic Details
Main Authors: Ying-jie Yang, Ye Wang, Zhi-feng Li, Ya Gong, Peng Zhang, Wen-chao Hu, Duo-hong Sheng, Yue-zhong Li
Format: Article
Language:English
Published: BMC 2017-08-01
Series:Microbial Cell Factories
Subjects:
CRISPR/Cas9
On-target cleavage efficiency
Spacer sequence
Free energy
Deletion of large genome fragments
Biosynthetic gene clusters for secondary metabolites
Online Access:http://link.springer.com/article/10.1186/s12934-017-0758-x

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http://link.springer.com/article/10.1186/s12934-017-0758-x

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