Detection and enumeration of Lak megaphages in microbiome samples by endpoint and quantitative PCR

Summary: Lak megaphages are prevalent across diverse gut microbiomes and may potentially impact animal and human health through lysis of Prevotella. Given their large genome size (up to 660 kbp), Lak megaphages are difficult to culture, and their identification relies on molecular techniques. Here,...

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Bibliographic Details
Main Authors: Marco A. Crisci, Paula M. Corsini, Nicola Bordin, Lin-Xing Chen, Jillain F. Banfield, Joanne M. Santini
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007358