PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

Abstract We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed r...

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Bibliographic Details
Main Authors: B. Molina-Moya, A. Lacoma, N. García-Sierra, S. Blanco, L. Haba, S. Samper, J. Ruiz-Manzano, C. Prat, C. Arnold, J. Domínguez
Format: Article
Language:English
Published: Nature Portfolio 2017-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-06760-5