Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids

As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on fluorescence resonance energy transfer (FRET). T...

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Váldodahkkit: Robert H. Batchelor, Adam Sarkez, W. Gregory Cox, Iain Johnson
Materiálatiipa: Artihkal
Giella:English
Almmustuhtton: Taylor & Francis Group 2007-10-01
Ráidu:BioTechniques
Liŋkkat:https://www.future-science.com/doi/10.2144/000112564