Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on fluorescence resonance energy transfer (FRET). T...
Váldodahkkit: | , , , |
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Materiálatiipa: | Artihkal |
Giella: | English |
Almmustuhtton: |
Taylor & Francis Group
2007-10-01
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Ráidu: | BioTechniques |
Liŋkkat: | https://www.future-science.com/doi/10.2144/000112564 |